BD PharmingenTM FITC Rat Anti-Mouse TCR Vbeta7

Description

Multicolor flow cytometric analysis of TCR Vbeta7 expression on Mouse peripheral T lymphocytes. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then simultaneously stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565 and with either FITC Rat IgG2b, kappa Isotype Control Cat. No. 556923 or FITC Rat Anti-Mouse TCR Vbeta7 antibody Cat. No. 570991 at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vbeta7 versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vbeta7 expression on Mouse peripheral T lymphocytes. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then simultaneously stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565 and with either FITC Rat IgG2b, kappa Isotype Control Cat. No. 556923 or FITC Rat Anti-Mouse TCR Vbeta7 antibody Cat. No. 570991 at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vbeta7 versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vbeta7 expression on Mouse peripheral T lymphocytes. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then simultaneously stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565 and with either FITC Rat IgG2b, kappa Isotype Control Cat. No. 556923 or FITC Rat Anti-Mouse TCR Vbeta7 antibody Cat. No. 570991 at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vbeta7 versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of TCR Vbeta7 expression on Mouse peripheral T lymphocytes. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then simultaneously stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565 and with either FITC Rat IgG2b, kappa Isotype Control Cat. No. 556923 or FITC Rat Anti-Mouse TCR Vbeta7 antibody Cat. No. 570991 at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vbeta7 versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of TCR Vbeta7 expression on Mouse peripheral T lymphocytes. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then simultaneously stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565 and with either FITC Rat IgG2b, kappa Isotype Control Cat. No. 556923 or FITC Rat Anti-Mouse TCR Vbeta7 antibody Cat. No. 570991 at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vbeta7 versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of TCR Vbeta7 expression on Mouse peripheral T lymphocytes. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then simultaneously stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565 and with either FITC Rat IgG2b, kappa Isotype Control Cat. No. 556923 or FITC Rat Anti-Mouse TCR Vbeta7 antibody Cat. No. 570991 at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of TCR Vbeta7 versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.