BD PharmingenTM HiCK-2 Human Cytokine Positive Control Cells

Description

Flow cytometric staining of HiCK-2 Human Cytokine Positive Control Cells for IL-3, IL-4, IL-10, IL-13 and GM-CSF . HiCK-2 cells were washed, permeabilized and subsequently stained with either a PE conjugated isotype control upper far left panel , PE Rat Anti-Human IL-3 Cat. No. 554676, upper left middle panel , PE Rat Anti-Human IL-4 Cat. No. 554516, upper right middle panel , PE Rat Anti-Human IL-10 Cat. No. 554706, upper far right panel , PE Rat Anti-Human IL-13 Cat. No. 554571, lower left panel or PE Rat Anti-Human GM-CSF Cat. No. 554507; lower middle panel . Despite fixation and freezing, the side- and forward-scattered light signals for these control cells see lower right panel remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown. Flow cytometric staining of HiCK-2 Human Cytokine Positive Control Cells for IL-3, IL-4, IL-10, IL-13 and GM-CSF . HiCK-2 cells were washed, permeabilized and subsequently stained with either a PE conjugated isotype control upper far left panel , PE Rat Anti-Human IL-3 Cat. No. 554676, upper left middle panel , PE Rat Anti-Human IL-4 Cat. No. 554516, upper right middle panel , PE Rat Anti-Human IL-10 Cat. No. 554706, upper far right panel , PE Rat Anti-Human IL-13 Cat. No. 554571, lower left panel or PE Rat Anti-Human GM-CSF Cat. No. 554507; lower middle panel . Despite fixation and freezing, the side- and forward-scattered light signals for these control cells see lower right panel remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown. Show More Flow cytometric staining of HiCK-2 Human Cytokine Positive Control Cells for IL-3, IL-4, IL-10, IL-13 and GM-CSF . HiCK-2 cells were washed, permeabilized and subsequently stained with either a PE conjugated isotype control upper far left panel , PE Rat Anti-Human IL-3 Cat. No. 554676, upper left middle panel , PE Rat Anti-Human IL-4 Cat. No. 554516, upper right middle panel , PE Rat Anti-Human IL-10 Cat. No. 554706, upper far right panel , PE Rat Anti-Human IL-13 Cat. No. 554571, lower left panel or PE Rat Anti-Human GM-CSF Cat. No. 554507; lower middle panel . Despite fixation and freezing, the side- and forward-scattered light signals for these control cells see lower right panel remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown.