BD PharmingenTM Human BD Fc BlockTM

Description

Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with BD Pharmingen Human BD Fc Block. Human peripheral blood mononuclear cells were either not treated dashed line histogram or preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/ 564220; solid line histogram . The cells were then stained with an excess of irrelevant PE-conjugated Mouse IgG2a antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with BD Pharmingen Human BD Fc Block. Human peripheral blood mononuclear cells were either not treated dashed line histogram or preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/ 564220; solid line histogram . The cells were then stained with an excess of irrelevant PE-conjugated Mouse IgG2a antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Show More Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with BD Pharmingen Human BD Fc Block. Human peripheral blood mononuclear cells were either not treated dashed line histogram or preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/ 564220; solid line histogram . The cells were then stained with an excess of irrelevant PE-conjugated Mouse IgG2a antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System.