BD PharmingenTM Human Lineage Cocktail 4 (lin 4) (CD2, CD3, CD4, CD7, CD8, CD10, CD11b, CD14, CD19, CD20, CD56, CD235a)

Description

Lineage cocktail 4 staining on enriched human cord blood: Frozen human cord blood mononuclear cells were thawed then enriched using the Human Lineage Cell Depletion Set Cat No. 560030 . The enriched human cord blood was then stained with the human lineage cocktail 4, APC CD34 Cat. No 340667 , BD Horizon BV421 CD38 Cat. No 562444 , APC-H7 CD45RA Cat. No. 560674 , BD Horizon BV605 CD90 Cat. No. 562685 and the viability dye 7-AAD Cat. No.559925 . Prospective hematopoietic stem cells HSCs were identified by first selecting cells based on the scatter profile left plot , then the Lineage negative and viable cells center left plot , then the CD34 + CD38 - cells center right plot and then the CD90 + CD45RA - cells right plot . Flow cytometry was performed on a BD LSRFortessa flow cytometry system. Lineage cocktail 4 staining on enriched human cord blood: Frozen human cord blood mononuclear cells were thawed then enriched using the Human Lineage Cell Depletion Set Cat No. 560030 . The enriched human cord blood was then stained with the human lineage cocktail 4, APC CD34 Cat. No 340667 , BD Horizon BV421 CD38 Cat. No 562444 , APC-H7 CD45RA Cat. No. 560674 , BD Horizon BV605 CD90 Cat. No. 562685 and the viability dye 7-AAD Cat. No.559925 . Prospective hematopoietic stem cells HSCs were identified by first selecting cells based on the scatter profile left plot , then the Lineage negative and viable cells center left plot , then the CD34 + CD38 - cells center right plot and then the CD90 + CD45RA - cells right plot . Flow cytometry was performed on a BD LSRFortessa flow cytometry system. Show More Lineage cocktail 4 staining on enriched human cord blood: Frozen human cord blood mononuclear cells were thawed then enriched using the Human Lineage Cell Depletion Set Cat No. 560030 . The enriched human cord blood was then stained with the human lineage cocktail 4, APC CD34 Cat. No 340667 , BD Horizon BV421 CD38 Cat. No 562444 , APC-H7 CD45RA Cat. No. 560674 , BD Horizon BV605 CD90 Cat. No. 562685 and the viability dye 7-AAD Cat. No.559925 . Prospective hematopoietic stem cells HSCs were identified by first selecting cells based on the scatter profile left plot , then the Lineage negative and viable cells center left plot , then the CD34 + CD38 - cells center right plot and then the CD90 + CD45RA - cells right plot . Flow cytometry was performed on a BD LSRFortessa flow cytometry system.