BD PharmingenTM Human Naive/Memory T Cell Panel

Description

Multicolor staining and flow cytometric analysis of peripheral blood naive and memory CD4+ T-cells using BD FACSCalibur and BD FACSCanto Cytometry Systems . Whole blood was stained with a combination of PerCP-Cy5.5 Mouse anti-Human CD4, FITC Mouse anti-Human CD45RA, and Alexa Fluor 647 Mouse anti-Human CD197 CCR7 antibodies for analysis with the BD FACSCalibur Cytometer System, or with the addition of APC-H7 Mouse anti-Human CD3 antibody, for analysis with the BD FACSCanto Cytometer System. The fluorescent antibodies provided in this kit were each used at 5 mul per test. Red blood cells were then lysed using BD Pharm Lyse Lysing Buffer Cat. No. 555899 and the leukocytes were analyzed. Bivariate dot plots showing the correlated expression patterns of CD45RA versus CD197 Panels A and B, Lower Plots were derived from gated events with the light-scattering characteristics of viable CD4+ T lymphocytes Panel A, Upper Plot, FACSCalibur or CD4+CD3+ T lymphocytes Panel B, Upper Plots, FACSCanto II that were generated by flow cytometric analysis. Quadrants for the dot plots were derived using fluorescence-minus-one FMO controls. Multicolor staining and flow cytometric analysis of peripheral blood naive and memory CD4+ T-cells using BD FACSCalibur and BD FACSCanto Cytometry Systems . Whole blood was stained with a combination of PerCP-Cy5.5 Mouse anti-Human CD4, FITC Mouse anti-Human CD45RA, and Alexa Fluor 647 Mouse anti-Human CD197 CCR7 antibodies for analysis with the BD FACSCalibur Cytometer System, or with the addition of APC-H7 Mouse anti-Human CD3 antibody, for analysis with the BD FACSCanto Cytometer System. The fluorescent antibodies provided in this kit were each used at 5 mul per test. Red blood cells were then lysed using BD Pharm Lyse Lysing Buffer Cat. No. 555899 and the leukocytes were analyzed. Bivariate dot plots showing the correlated expression patterns of CD45RA versus CD197 Panels A and B, Lower Plots were derived from gated events with the light-scattering characteristics of viable CD4+ T lymphocytes Panel A, Upper Plot, FACSCalibur or CD4+CD3+ T lymphocytes Panel B, Upper Plots, FACSCanto II that were generated by flow cytometric analysis. Quadrants for the dot plots were derived using fluorescence-minus-one FMO controls. Show More Multicolor staining and flow cytometric analysis of peripheral blood naive and memory CD4+ T-cells using BD FACSCalibur and BD FACSCanto Cytometry Systems . Whole blood was stained with a combination of PerCP-Cy5.5 Mouse anti-Human CD4, FITC Mouse anti-Human CD45RA, and Alexa Fluor 647 Mouse anti-Human CD197 CCR7 antibodies for analysis with the BD FACSCalibur Cytometer System, or with the addition of APC-H7 Mouse anti-Human CD3 antibody, for analysis with the BD FACSCanto Cytometer System. The fluorescent antibodies provided in this kit were each used at 5 mul per test. Red blood cells were then lysed using BD Pharm Lyse Lysing Buffer Cat. No. 555899 and the leukocytes were analyzed. Bivariate dot plots showing the correlated expression patterns of CD45RA versus CD197 Panels A and B, Lower Plots were derived from gated events with the light-scattering characteristics of viable CD4+ T lymphocytes Panel A, Upper Plot, FACSCalibur or CD4+CD3+ T lymphocytes Panel B, Upper Plots, FACSCanto II that were generated by flow cytometric analysis. Quadrants for the dot plots were derived using fluorescence-minus-one FMO controls.