BD PharmingenTM MiCK-1 Mouse Cytokine Positive Control Cells

Description

Characteristic staining of MiCK-1 Positive Control Cells with IL-2, TNF, and IFN-gamma . MiCK-1 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control PE-R3-34; Cat. No. 554685; see Panel A , PE-rat anti-mouse IL-2 antibody PE-JES6-5H4, Cat. No. 554428 see Panel B , PE-rat anti-mouse TNF PE-MP6-XT22, Cat. No. 554419; Panel C , and PE-rat anti-mouse IFN-gamma PE-XMG1.2, Cat. No. 554412; Panel D . Despite fixation and freezing, the side- and forward-scattered light signals for these control cells see Panel E remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Bivariate markers for the gated monocyte population Panel E were set based on autofluorescence and isotype controls. The percentage of cytokine expressing cells was calculated based on the gated monocyte cell population shown in Panel E. Characteristic staining of MiCK-1 Positive Control Cells with IL-2, TNF, and IFN-gamma . MiCK-1 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control PE-R3-34; Cat. No. 554685; see Panel A , PE-rat anti-mouse IL-2 antibody PE-JES6-5H4, Cat. No. 554428 see Panel B , PE-rat anti-mouse TNF PE-MP6-XT22, Cat. No. 554419; Panel C , and PE-rat anti-mouse IFN-gamma PE-XMG1.2, Cat. No. 554412; Panel D . Despite fixation and freezing, the side- and forward-scattered light signals for these control cells see Panel E remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Bivariate markers for the gated monocyte population Panel E were set based on autofluorescence and isotype controls. The percentage of cytokine expressing cells was calculated based on the gated monocyte cell population shown in Panel E. Show More Characteristic staining of MiCK-1 Positive Control Cells with IL-2, TNF, and IFN-gamma . MiCK-1 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control PE-R3-34; Cat. No. 554685; see Panel A , PE-rat anti-mouse IL-2 antibody PE-JES6-5H4, Cat. No. 554428 see Panel B , PE-rat anti-mouse TNF PE-MP6-XT22, Cat. No. 554419; Panel C , and PE-rat anti-mouse IFN-gamma PE-XMG1.2, Cat. No. 554412; Panel D . Despite fixation and freezing, the side- and forward-scattered light signals for these control cells see Panel E remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Bivariate markers for the gated monocyte population Panel E were set based on autofluorescence and isotype controls. The percentage of cytokine expressing cells was calculated based on the gated monocyte cell population shown in Panel E.