BD PharmingenTM MiCK-2 Mouse Cytokine Positive Control Cells

Description

Characteristic staining of MiCK-2 Positive Control Cells with anti-mouse IL-4, IL-10 and GM-CSF. MiCK-2 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control PE-R3-34; Cat. No. 554685; Panel A , PE-rat anti-mouse IL-4 PE-11B11, Cat. No. 554435; Panel B , PE-rat anti-mouse IL-10 PE-JES5-16E3, Cat. No. 554467; Panel C or PE-rat anti-mouse GM-CSF PE-MP1-22E9, Cat. No. 554406; Panel D . Despite fixation and freezing, the side-and forward-scattered light signals for these control cells Panel F remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown in Panels A-D. Characteristic staining of MiCK-2 Positive Control Cells with anti-mouse IL-4, IL-10 and GM-CSF. MiCK-2 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control PE-R3-34; Cat. No. 554685; Panel A , PE-rat anti-mouse IL-4 PE-11B11, Cat. No. 554435; Panel B , PE-rat anti-mouse IL-10 PE-JES5-16E3, Cat. No. 554467; Panel C or PE-rat anti-mouse GM-CSF PE-MP1-22E9, Cat. No. 554406; Panel D . Despite fixation and freezing, the side-and forward-scattered light signals for these control cells Panel F remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown in Panels A-D. Show More Characteristic staining of MiCK-2 Positive Control Cells with anti-mouse IL-4, IL-10 and GM-CSF. MiCK-2 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control PE-R3-34; Cat. No. 554685; Panel A , PE-rat anti-mouse IL-4 PE-11B11, Cat. No. 554435; Panel B , PE-rat anti-mouse IL-10 PE-JES5-16E3, Cat. No. 554467; Panel C or PE-rat anti-mouse GM-CSF PE-MP1-22E9, Cat. No. 554406; Panel D . Despite fixation and freezing, the side-and forward-scattered light signals for these control cells Panel F remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Quadrant markers were set based on the autofluorescence controls to calculate the percentages of cells contained in each quadrant region as shown in Panels A-D.