BD PharmingenTM MiCK-3 Mouse Cytokine Positive Control Cells

Description

Characteristic staining of MiCK-3 Positive Control Cells with anti-mouse IL-1alpha, IL-6, IL-12, MCP-1 and TNF. MiCK-3 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control PE-R3-34; Cat. No. 554685; see Panel A , PE-hamster anti-mouse IL-1alpha antibody PE-ALF-161, Cat. No. 559810, see Panel B , PE-rat anti-mouse IL-6 antibody PE-MP5-20F3, Cat. No. 554401, see Panel C , PE-rat anti-mouse IL-12 p40/p70 PE-C15.6, Cat. No. 554479; Panel D , PE-hamster anti-mouse MCP-1 PE-2H5, Cat. No. 554443; Panel E or PE-rat anti-mouse TNF PE-MP6-XT22, Cat. No. 554419; Panel F . Despite fixation and freezing, the side- and forward-scattered light signals for these control cells see Panel G remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Quadrant markers were set based on the autofluorescence controls and were verified using the recombinant cytokine blocking and unlabeled antibody blocking specificity controls, data not shown to calculate the percentages of cells contained in each quadrant region as shown in Panels A-F. Characteristic staining of MiCK-3 Positive Control Cells with anti-mouse IL-1alpha, IL-6, IL-12, MCP-1 and TNF. MiCK-3 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control PE-R3-34; Cat. No. 554685; see Panel A , PE-hamster anti-mouse IL-1alpha antibody PE-ALF-161, Cat. No. 559810, see Panel B , PE-rat anti-mouse IL-6 antibody PE-MP5-20F3, Cat. No. 554401, see Panel C , PE-rat anti-mouse IL-12 p40/p70 PE-C15.6, Cat. No. 554479; Panel D , PE-hamster anti-mouse MCP-1 PE-2H5, Cat. No. 554443; Panel E or PE-rat anti-mouse TNF PE-MP6-XT22, Cat. No. 554419; Panel F . Despite fixation and freezing, the side- and forward-scattered light signals for these control cells see Panel G remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Quadrant markers were set based on the autofluorescence controls and were verified using the recombinant cytokine blocking and unlabeled antibody blocking specificity controls, data not shown to calculate the percentages of cells contained in each quadrant region as shown in Panels A-F. Show More Characteristic staining of MiCK-3 Positive Control Cells with anti-mouse IL-1alpha, IL-6, IL-12, MCP-1 and TNF. MiCK-3 cells were washed, permeabilized, and subsequently stained with PE-rat IgG1 isotype control PE-R3-34; Cat. No. 554685; see Panel A , PE-hamster anti-mouse IL-1alpha antibody PE-ALF-161, Cat. No. 559810, see Panel B , PE-rat anti-mouse IL-6 antibody PE-MP5-20F3, Cat. No. 554401, see Panel C , PE-rat anti-mouse IL-12 p40/p70 PE-C15.6, Cat. No. 554479; Panel D , PE-hamster anti-mouse MCP-1 PE-2H5, Cat. No. 554443; Panel E or PE-rat anti-mouse TNF PE-MP6-XT22, Cat. No. 554419; Panel F . Despite fixation and freezing, the side- and forward-scattered light signals for these control cells see Panel G remain similar to those for freshly-prepared lymphoid cell preparations data not shown . Quadrant markers were set based on the autofluorescence controls and were verified using the recombinant cytokine blocking and unlabeled antibody blocking specificity controls, data not shown to calculate the percentages of cells contained in each quadrant region as shown in Panels A-F.