BD PharmingenTM MonoBlockTM Leukocyte Staining Buffer

Description

Figure 1. BD Pharmingen MonoBlock Leukocyte Staining Buffer reduces nonspecific staining of leukocytes. Human leukocytes were stained with either MOPC-21 or X-40 Mouse IgG1, kappa Isotype Controls mIgG1, kappa Iso Ctrl conjugated with either APC-Cy7, APC-H7, PE-Cy5, PE-Cy7, or PE-CF594. These controls were either not premixed Upper Plots or premixed Lower Plots with BD Pharmingen MonoBlock Leukocyte Staining Buffer as indicated. The cells were washed and analyzed. Bivariate pseudocolor density plots show reduced background staining on leukocyte that were stained in the presence of this buffer. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Figure 2. BD Pharmingen MonoBlock Leukocyte Staining Buffer reduces nonspecific staining but preserves specific staining of human leukocytes with PE-Cy7 antibodies. PE-Cy7-conjugated clones specific for human CD14 M5E2 , CD33 P67.6 , CD64 10.1 , CD279/PD-1 EH12.1 , CD3 UCHT1 , CD19 HIB19 either were not mixed Upper Plots or were mixed Lower Plots with BD Pharmingen MonoBlock Leukocyte Staining Buffer. After staining leukocytes with these, they were washed and analyzed. The plots show reduced background staining on leukocytes that were stained using this buffer. Figure 3. BD Pharmingen MonoBlock Leukocyte Staining Buffer reduces nonspecific staining but preserves specific staining of mouse bone marrow cells BMC for CD11b. Mouse BMC were either not preincubated Top Plots or were preincubated Middle, Bottom Plots with BD Pharmingen Mouse BD Fc Block as indicated. PE-Cy7 Rat IgG2b, kappa Isotype Control Left Plots or PE-Cy7 Rat Anti-CD11b Right Plots were either not premixed Top and Middle Plots or were mixed Bottom Plots with this buffer. The cells were then stained with the PE-Cy7- reagents, washed, and similarly analyzed by flow cytometry. Figure 1. BD Pharmingen MonoBlock Leukocyte Staining Buffer reduces nonspecific staining of leukocytes. Human leukocytes were stained with either MOPC-21 or X-40 Mouse IgG1, kappa Isotype Controls mIgG1, kappa Iso Ctrl conjugated with either APC-Cy7, APC-H7, PE-Cy5, PE-Cy7, or PE-CF594. These controls were either not premixed Upper Plots or premixed Lower Plots with BD Pharmingen MonoBlock Leukocyte Staining Buffer as indicated. The cells were washed and analyzed. Bivariate pseudocolor density plots show reduced background staining on leukocyte that were stained in the presence of this buffer. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Figure 2. BD Pharmingen MonoBlock Leukocyte Staining Buffer reduces nonspecific staining but preserves specific staining of human leukocytes with PE-Cy7 antibodies. PE-Cy7-conjugated clones specific for human CD14 M5E2 , CD33 P67.6 , CD64 10.1 , CD279/PD-1 EH12.1 , CD3 UCHT1 , CD19 HIB19 either were not mixed Upper Plots or were mixed Lower Plots with BD Pharmingen MonoBlock Leukocyte Staining Buffer. After staining leukocytes with these, they were washed and analyzed. The plots show reduced background staining on leukocytes that were stained using this buffer. Figure 3. BD Pharmingen MonoBlock Leukocyte Staining Buffer reduces nonspecific staining but preserves specific staining of mouse bone marrow cells BMC for CD11b. Mouse BMC were either not preincubated Top Plots or were preincubated Middle, Bottom Plots with BD Pharmingen Mouse BD Fc Block as indicated. PE-Cy7 Rat IgG2b, kappa Isotype Control Left Plots or PE-Cy7 Rat Anti-CD11b Right Plots were either not premixed Top and Middle Plots or were mixed Bottom Plots with this buffer. The cells were then stained with the PE-Cy7- reagents, washed, and similarly analyzed by flow cytometry. Show More Figure 1. BD Pharmingen MonoBlock Leukocyte Staining Buffer reduces nonspecific staining of leukocytes. Human leukocytes were stained with either MOPC-21 or X-40 Mouse IgG1, kappa Isotype Controls mIgG1, kappa Iso Ctrl conjugated with either APC-Cy7, APC-H7, PE-Cy5, PE-Cy7, or PE-CF594. These controls were either not premixed Upper Plots or premixed Lower Plots with BD Pharmingen MonoBlock Leukocyte Staining Buffer as indicated. The cells were washed and analyzed. Bivariate pseudocolor density plots show reduced background staining on leukocyte that were stained in the presence of this buffer. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Figure 2. BD Pharmingen MonoBlock Leukocyte Staining Buffer reduces nonspecific staining but preserves specific staining of human leukocytes with PE-Cy7 antibodies. PE-Cy7-conjugated clones specific for human CD14 M5E2 , CD33 P67.6 , CD64 10.1 , CD279/PD-1 EH12.1 , CD3 UCHT1 , CD19 HIB19 either were not mixed Upper Plots or were mixed Lower Plots with BD Pharmingen MonoBlock Leukocyte Staining Buffer. After staining leukocytes with these, they were washed and analyzed. The plots show reduced background staining on leukocytes that were stained using this buffer. Figure 3. BD Pharmingen MonoBlock Leukocyte Staining Buffer reduces nonspecific staining but preserves specific staining of mouse bone marrow cells BMC for CD11b. Mouse BMC were either not preincubated Top Plots or were preincubated Middle, Bottom Plots with BD Pharmingen Mouse BD Fc Block as indicated. PE-Cy7 Rat IgG2b, kappa Isotype Control Left Plots or PE-Cy7 Rat Anti-CD11b Right Plots were either not premixed Top and Middle Plots or were mixed Bottom Plots with this buffer. The cells were then stained with the PE-Cy7- reagents, washed, and similarly analyzed by flow cytometry.