BD PharmingenTM Mouse B Lymphocyte Activation Antibody Cocktail, with Isotype Control; PE-CyTM7 CD25, PE CD69, & FITC CD19

Description

Identification of activated B lymphocytes using Mouse B Lymphocyte Activation Antibody Cocktail, with Isotype Control. BALB/c splenocytes were activated by culture for 48 hours with anti-IgM antibody Jackson immunoresearch and stained with either Mouse B Lymphocyte Activation Isotype Control left panels or Mouse B Lymphocyte Activation Antibody Cocktail middle panels . Unactivated BALB/c splenocytes were stained with Mouse B Lymphocyte Activation Antibody Cocktail right panels or Mouse B Lymphocyte Activation Isotype Control not shown . Scatter plots were used to select either activated lymphoblasts left and middle panels or resting lymphocytes right panels for data analysis. The two-color contour plots display the CD19+ B lymphocytes which express the activation antigens CD25 top of middle and right panels and CD69 bottom of middle and right panels . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Identification of activated B lymphocytes using Mouse B Lymphocyte Activation Antibody Cocktail, with Isotype Control. BALB/c splenocytes were activated by culture for 48 hours with anti-IgM antibody Jackson immunoresearch and stained with either Mouse B Lymphocyte Activation Isotype Control left panels or Mouse B Lymphocyte Activation Antibody Cocktail middle panels . Unactivated BALB/c splenocytes were stained with Mouse B Lymphocyte Activation Antibody Cocktail right panels or Mouse B Lymphocyte Activation Isotype Control not shown . Scatter plots were used to select either activated lymphoblasts left and middle panels or resting lymphocytes right panels for data analysis. The two-color contour plots display the CD19+ B lymphocytes which express the activation antigens CD25 top of middle and right panels and CD69 bottom of middle and right panels . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Identification of activated B lymphocytes using Mouse B Lymphocyte Activation Antibody Cocktail, with Isotype Control. BALB/c splenocytes were activated by culture for 48 hours with anti-IgM antibody Jackson immunoresearch and stained with either Mouse B Lymphocyte Activation Isotype Control left panels or Mouse B Lymphocyte Activation Antibody Cocktail middle panels . Unactivated BALB/c splenocytes were stained with Mouse B Lymphocyte Activation Antibody Cocktail right panels or Mouse B Lymphocyte Activation Isotype Control not shown . Scatter plots were used to select either activated lymphoblasts left and middle panels or resting lymphocytes right panels for data analysis. The two-color contour plots display the CD19+ B lymphocytes which express the activation antigens CD25 top of middle and right panels and CD69 bottom of middle and right panels . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.