BD PharmingenTM Mouse T Lymphocyte Activation Antibody Cocktail, with Isotype Control PE-CyTM7 CD25, PE CD69, and APC CD3e

Description

Identification of activated T lymphocytes using Mouse T Lymphocyte Activation Antibody Cocktail, with Isotype Control. BALB/c splenocytes were activated in culture for 48 hours on plate-bound Purified Hamster Anti-Mouse CD3e Clone 500A2; Cat. No. 553238 and scatter plots were used to select either activated lymphoblasts Left and Middle Panels or resting unactivated lymphocytes Right Panels for data analysis. The activated splenocytes were stained with either Mouse T Lymphocyte Activation Isotype Control Left Panels or Mouse T Lymphocyte Activation Antibody Cocktail Middle Panels . Resting splenocytes were stained with Mouse T Lymphocyte Activation Antibody Cocktail Right Panels or Mouse T Lymphocyte Activation Isotype Control not shown . The two-color contour plots display the CD3e+ activated lymphoblasts which have upregulated CD25 Middle Top Panel and CD69 Middle Bottom Panel antigen expression. Flow cytometric analysis was performed on a BD FACSCalibur flow cytometry system. Identification of activated T lymphocytes using Mouse T Lymphocyte Activation Antibody Cocktail, with Isotype Control. BALB/c splenocytes were activated in culture for 48 hours on plate-bound Purified Hamster Anti-Mouse CD3e Clone 500A2; Cat. No. 553238 and scatter plots were used to select either activated lymphoblasts Left and Middle Panels or resting unactivated lymphocytes Right Panels for data analysis. The activated splenocytes were stained with either Mouse T Lymphocyte Activation Isotype Control Left Panels or Mouse T Lymphocyte Activation Antibody Cocktail Middle Panels . Resting splenocytes were stained with Mouse T Lymphocyte Activation Antibody Cocktail Right Panels or Mouse T Lymphocyte Activation Isotype Control not shown . The two-color contour plots display the CD3e+ activated lymphoblasts which have upregulated CD25 Middle Top Panel and CD69 Middle Bottom Panel antigen expression. Flow cytometric analysis was performed on a BD FACSCalibur flow cytometry system. Show More Identification of activated T lymphocytes using Mouse T Lymphocyte Activation Antibody Cocktail, with Isotype Control. BALB/c splenocytes were activated in culture for 48 hours on plate-bound Purified Hamster Anti-Mouse CD3e Clone 500A2; Cat. No. 553238 and scatter plots were used to select either activated lymphoblasts Left and Middle Panels or resting unactivated lymphocytes Right Panels for data analysis. The activated splenocytes were stained with either Mouse T Lymphocyte Activation Isotype Control Left Panels or Mouse T Lymphocyte Activation Antibody Cocktail Middle Panels . Resting splenocytes were stained with Mouse T Lymphocyte Activation Antibody Cocktail Right Panels or Mouse T Lymphocyte Activation Isotype Control not shown . The two-color contour plots display the CD3e+ activated lymphoblasts which have upregulated CD25 Middle Top Panel and CD69 Middle Bottom Panel antigen expression. Flow cytometric analysis was performed on a BD FACSCalibur flow cytometry system.