BD PharmingenTM Pacific BlueTM Rat Anti-Mouse CD8a

Description

Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either Pacific Blue Rat IgG2a, kappa Isotype Control Cat. No. 558109; Left Plot or Pacific Blue Rat Anti-Mouse CD8a Cat. No. 558106; Right Plot antibodies at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either Pacific Blue Rat IgG2a, kappa Isotype Control Cat. No. 558109; Left Plot or Pacific Blue Rat Anti-Mouse CD8a Cat. No. 558106; Right Plot antibodies at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either Pacific Blue Rat IgG2a, kappa Isotype Control Cat. No. 558109; Left Plot or Pacific Blue Rat Anti-Mouse CD8a Cat. No. 558106; Right Plot antibodies at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either Pacific Blue Rat IgG2a, kappa Isotype Control Cat. No. 558109; Left Plot or Pacific Blue Rat Anti-Mouse CD8a Cat. No. 558106; Right Plot antibodies at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either Pacific Blue Rat IgG2a, kappa Isotype Control Cat. No. 558109; Left Plot or Pacific Blue Rat Anti-Mouse CD8a Cat. No. 558106; Right Plot antibodies at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either Pacific Blue Rat IgG2a, kappa Isotype Control Cat. No. 558109; Left Plot or Pacific Blue Rat Anti-Mouse CD8a Cat. No. 558106; Right Plot antibodies at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.