BD PharmingenTM PE Active Caspase-3 Apoptosis Kit

Description

Flow cytometric analysis of Active Caspase-3 expression by Untreated or Camptothecin-treated Human Jurkat cells. Cells from the human Jurkat T-cell leukemia, ATCC TIB-152 cell line were cultured 6 hrs without Untreated; Left histogram or with 12 muM of camptothecin Camptothecin-treated; Right histogram to induce apoptosis. Cells were permeabilized, fixed, and stained for Active Caspase-3 as described in the accompanying Staining Protocol. The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scattered characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of Active Caspase-3 expression by Untreated or Dexamethasone-treated Mouse thymocytes. C57BL/6 thymocytes were cultured 5 hours without Untreated; Left histogram or with 1 muM of dexamethasone Dexamethasone-treated; Right histogram for 5 hours to induce apoptosis. Cells were permeabilized, fixed, and stained for Active Caspase-3 as described in the accompanying Staining Protocol. The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scattered characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of Active Caspase-3 expression by Untreated or Camptothecin-treated Human Jurkat cells. Cells from the human Jurkat T-cell leukemia, ATCC TIB-152 cell line were cultured 6 hrs without Untreated; Left histogram or with 12 muM of camptothecin Camptothecin-treated; Right histogram to induce apoptosis. Cells were permeabilized, fixed, and stained for Active Caspase-3 as described in the accompanying Staining Protocol. The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scattered characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of Active Caspase-3 expression by Untreated or Dexamethasone-treated Mouse thymocytes. C57BL/6 thymocytes were cultured 5 hours without Untreated; Left histogram or with 1 muM of dexamethasone Dexamethasone-treated; Right histogram for 5 hours to induce apoptosis. Cells were permeabilized, fixed, and stained for Active Caspase-3 as described in the accompanying Staining Protocol. The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scattered characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of Active Caspase-3 expression by Untreated or Camptothecin-treated Human Jurkat cells. Cells from the human Jurkat T-cell leukemia, ATCC TIB-152 cell line were cultured 6 hrs without Untreated; Left histogram or with 12 muM of camptothecin Camptothecin-treated; Right histogram to induce apoptosis. Cells were permeabilized, fixed, and stained for Active Caspase-3 as described in the accompanying Staining Protocol. The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scattered characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of Active Caspase-3 expression by Untreated or Dexamethasone-treated Mouse thymocytes. C57BL/6 thymocytes were cultured 5 hours without Untreated; Left histogram or with 1 muM of dexamethasone Dexamethasone-treated; Right histogram for 5 hours to induce apoptosis. Cells were permeabilized, fixed, and stained for Active Caspase-3 as described in the accompanying Staining Protocol. The fluorescence histograms showing Active Caspase-3 expression were derived from gated events with the forward and side light-scattered characteristics of single cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.