BD PharmingenTM PE Annexin V

Description

PE Annexin V: A tool for identifying cells that are undergoing apoptosis. Jurkat T cells were left untreated upper left & lower left panels , treated for 5 hours upper middle & lower middle panels or 12 hours upper right & lower right panels with anti-human Fas antibody clone DX2, Cat. No. 555670 and Protein G. Cells were incubated with PE Annexin V in a buffer containing 7-amino-actinomycin 7-AAD, Cat. No. 559925 and analyzed by flow cytometry. Untreated cells were primarily PE Annexin V and 7-AAD negative, indicating that they were viable and not undergoing apoptosis. After a 5 hour treatment with DX2, there were two populations of cells: cells undergoing apoptosis PE Annexin V positive and 7-AAD negative , and cells that were viable and not undergoing apoptosis PE Annexin V and 7-AAD negative . After a 12 hour treatment with DX2, three populations of cells were identified: Cells that had died or were in latest stage of apoptosis PE Annexin V and 7-AAD positive , cells undergoing apoptosis PE Annexin V positive and 7-AAD negative , and cells that were viable and not undergoing apoptosis PE Annexin V and 7-AAD negative . PE Annexin V: A tool for identifying cells that are undergoing apoptosis. Jurkat T cells were left untreated upper left & lower left panels , treated for 5 hours upper middle & lower middle panels or 12 hours upper right & lower right panels with anti-human Fas antibody clone DX2, Cat. No. 555670 and Protein G. Cells were incubated with PE Annexin V in a buffer containing 7-amino-actinomycin 7-AAD, Cat. No. 559925 and analyzed by flow cytometry. Untreated cells were primarily PE Annexin V and 7-AAD negative, indicating that they were viable and not undergoing apoptosis. After a 5 hour treatment with DX2, there were two populations of cells: cells undergoing apoptosis PE Annexin V positive and 7-AAD negative , and cells that were viable and not undergoing apoptosis PE Annexin V and 7-AAD negative . After a 12 hour treatment with DX2, three populations of cells were identified: Cells that had died or were in latest stage of apoptosis PE Annexin V and 7-AAD positive , cells undergoing apoptosis PE Annexin V positive and 7-AAD negative , and cells that were viable and not undergoing apoptosis PE Annexin V and 7-AAD negative . Show More PE Annexin V: A tool for identifying cells that are undergoing apoptosis. Jurkat T cells were left untreated upper left & lower left panels , treated for 5 hours upper middle & lower middle panels or 12 hours upper right & lower right panels with anti-human Fas antibody clone DX2, Cat. No. 555670 and Protein G. Cells were incubated with PE Annexin V in a buffer containing 7-amino-actinomycin 7-AAD, Cat. No. 559925 and analyzed by flow cytometry. Untreated cells were primarily PE Annexin V and 7-AAD negative, indicating that they were viable and not undergoing apoptosis. After a 5 hour treatment with DX2, there were two populations of cells: cells undergoing apoptosis PE Annexin V positive and 7-AAD negative , and cells that were viable and not undergoing apoptosis PE Annexin V and 7-AAD negative . After a 12 hour treatment with DX2, three populations of cells were identified: Cells that had died or were in latest stage of apoptosis PE Annexin V and 7-AAD positive , cells undergoing apoptosis PE Annexin V positive and 7-AAD negative , and cells that were viable and not undergoing apoptosis PE Annexin V and 7-AAD negative .