BD PharmingenTM PE Annexin V Apoptosis Detection Kit I

Description

Flow Cytometric Analysis of PE Annexin V staining. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated top panels or treated for 4 hours with 4 muM Camptothecin bottom panels . Cells were incubated with PE Annexin V in a buffer containing 7-Amino-Actinomycin 7-AAD and analyzed by flow cytometry. Untreated cells were primarily PE Annexin V and 7-AAD negative, indicating that they were viable and not undergoing apoptosis. After a 4 hour treatment bottom panels , there were primarily two populations of cells: Cells that were viable and not undergoing apoptosis PE Annexin V and 7-AAD negative ; cells undergoing apoptosis PE Annexin V positive and 7-AAD negative . A minor population of cells were observed to be PE Annexin V and 7-AAD positive, indicating that they were in end stage apoptosis or already dead. Flow Cytometric Analysis of PE Annexin V staining. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated top panels or treated for 4 hours with 4 muM Camptothecin bottom panels . Cells were incubated with PE Annexin V in a buffer containing 7-Amino-Actinomycin 7-AAD and analyzed by flow cytometry. Untreated cells were primarily PE Annexin V and 7-AAD negative, indicating that they were viable and not undergoing apoptosis. After a 4 hour treatment bottom panels , there were primarily two populations of cells: Cells that were viable and not undergoing apoptosis PE Annexin V and 7-AAD negative ; cells undergoing apoptosis PE Annexin V positive and 7-AAD negative . A minor population of cells were observed to be PE Annexin V and 7-AAD positive, indicating that they were in end stage apoptosis or already dead. Show More Flow Cytometric Analysis of PE Annexin V staining. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated top panels or treated for 4 hours with 4 muM Camptothecin bottom panels . Cells were incubated with PE Annexin V in a buffer containing 7-Amino-Actinomycin 7-AAD and analyzed by flow cytometry. Untreated cells were primarily PE Annexin V and 7-AAD negative, indicating that they were viable and not undergoing apoptosis. After a 4 hour treatment bottom panels , there were primarily two populations of cells: Cells that were viable and not undergoing apoptosis PE Annexin V and 7-AAD negative ; cells undergoing apoptosis PE Annexin V positive and 7-AAD negative . A minor population of cells were observed to be PE Annexin V and 7-AAD positive, indicating that they were in end stage apoptosis or already dead.