BD PharmingenTM PE Armenian Hamster Anti-Helios

Description

Two-color flow cytometric analysis of Helios expression Left Panel: Human peripheral blood mononuclear cells were stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat No. 562424/562425 and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Hamster IgG2, kappa Isotype Control Cat No. 550085, Left Plot or PE Armenian Hamster Anti-Helios antibody Cat. No. 563801; Right Plot . Right Panel: Mouse splenic leucocytes were stained with BD Horizon BV421 Rat Anti-Mouse CD4 antibody Cat No. 562891 and fixed and permeabilized with the BD Transcription Factor Buffer Set. The cells were then stained with either PE Hamster IgG2, kappa Isotype Control Left Plot or PE Armenian Hamster Anti-Helios antibody Right Plot . The two-color flow cytometric dot plots show the correlated expression patterns of Helios or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes or splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Two-color flow cytometric analysis of Helios expression Left Panel: Human peripheral blood mononuclear cells were stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat No. 562424/562425 and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Hamster IgG2, kappa Isotype Control Cat No. 550085, Left Plot or PE Armenian Hamster Anti-Helios antibody Cat. No. 563801; Right Plot . Right Panel: Mouse splenic leucocytes were stained with BD Horizon BV421 Rat Anti-Mouse CD4 antibody Cat No. 562891 and fixed and permeabilized with the BD Transcription Factor Buffer Set. The cells were then stained with either PE Hamster IgG2, kappa Isotype Control Left Plot or PE Armenian Hamster Anti-Helios antibody Right Plot . The two-color flow cytometric dot plots show the correlated expression patterns of Helios or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes or splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Show More Two-color flow cytometric analysis of Helios expression Left Panel: Human peripheral blood mononuclear cells were stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat No. 562424/562425 and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Hamster IgG2, kappa Isotype Control Cat No. 550085, Left Plot or PE Armenian Hamster Anti-Helios antibody Cat. No. 563801; Right Plot . Right Panel: Mouse splenic leucocytes were stained with BD Horizon BV421 Rat Anti-Mouse CD4 antibody Cat No. 562891 and fixed and permeabilized with the BD Transcription Factor Buffer Set. The cells were then stained with either PE Hamster IgG2, kappa Isotype Control Left Plot or PE Armenian Hamster Anti-Helios antibody Right Plot . The two-color flow cytometric dot plots show the correlated expression patterns of Helios or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes or splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System.