BD PharmingenTM PE-Cy7 Mouse Anti-Human CCR7 (CD197)

Description

Multiparameter flow cytometric analysis of CCR7 CD197 on Human peripheral blood leukocytes and lymphocytes. Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , FITC Mouse Anti-Human CD8 Cat. No. 561947/555366/557085 , BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 antibodies, and either PE-Cy7 Mouse IgG1, kappa Isotype Control Cat. No. 565573 or PE-Cy7 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 567313/567314 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa X20 Flow Cytometer and FlowJo software. Plots 1 and 2: Bivariate pseudocolor density plots showing Ig isotype control or CCR7 CD197 staining versus side light-scatter SSC-A were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: Bivariate pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: Bivariate pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Multiparameter flow cytometric analysis of CCR7 CD197 on Human peripheral blood leukocytes and lymphocytes. Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , FITC Mouse Anti-Human CD8 Cat. No. 561947/555366/557085 , BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 antibodies, and either PE-Cy7 Mouse IgG1, kappa Isotype Control Cat. No. 565573 or PE-Cy7 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 567313/567314 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa X20 Flow Cytometer and FlowJo software. Plots 1 and 2: Bivariate pseudocolor density plots showing Ig isotype control or CCR7 CD197 staining versus side light-scatter SSC-A were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: Bivariate pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: Bivariate pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Show More Multiparameter flow cytometric analysis of CCR7 CD197 on Human peripheral blood leukocytes and lymphocytes. Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , FITC Mouse Anti-Human CD8 Cat. No. 561947/555366/557085 , BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 antibodies, and either PE-Cy7 Mouse IgG1, kappa Isotype Control Cat. No. 565573 or PE-Cy7 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 567313/567314 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa X20 Flow Cytometer and FlowJo software. Plots 1 and 2: Bivariate pseudocolor density plots showing Ig isotype control or CCR7 CD197 staining versus side light-scatter SSC-A were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: Bivariate pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: Bivariate pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of intact lymphocytes.