BD PharmingenTM PE-Cy7 Mouse Anti-Human CD45

Description

Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either PE-Cy-7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD45 antibody Cat. No. 570765/570843; Right Plot in the presence of BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either PE-Cy-7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD45 antibody Cat. No. 570765/570843; Right Plot in the presence of BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either PE-Cy-7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD45 antibody Cat. No. 570765/570843; Right Plot in the presence of BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either PE-Cy-7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD45 antibody Cat. No. 570765/570843; Right Plot in the presence of BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either PE-Cy-7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD45 antibody Cat. No. 570765/570843; Right Plot in the presence of BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either PE-Cy-7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD45 antibody Cat. No. 570765/570843; Right Plot in the presence of BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.