BD PharmingenTM PE-Cy7 Mouse Anti-Human CD8

Description

Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leukocyte populations. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with Human whole blood followed by staining with either PE-Cy7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD8 antibody Cat. No. 570819/570895; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD8 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leukocyte populations. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with Human whole blood followed by staining with either PE-Cy7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD8 antibody Cat. No. 570819/570895; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD8 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leukocyte populations. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with Human whole blood followed by staining with either PE-Cy7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD8 antibody Cat. No. 570819/570895; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD8 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leukocyte populations. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with Human whole blood followed by staining with either PE-Cy7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD8 antibody Cat. No. 570819/570895; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD8 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leukocyte populations. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with Human whole blood followed by staining with either PE-Cy7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD8 antibody Cat. No. 570819/570895; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD8 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD8 expression on Human peripheral blood leukocyte populations. In order to reduce nonspecific background staining, BD Pharmingen MonoBlock Leukocyte Staining Buffer Cat. No. 570002 was added and mixed with Human whole blood followed by staining with either PE-Cy7 Mouse IgG1, kappa Isotype Control Cat. No. 565573; Left Plot or PE-Cy7 Mouse Anti-Human CD8 antibody Cat. No. 570819/570895; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD8 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.