BD PharmingenTM PE-Cy7 Mouse Anti-Human ICOS (CD278)

Description

Flow cytometric analysis of ICOS CD278 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either PE-Cy7 Mouse IgG1 kappa Isotype Control Cat. No. 565573; dashed line histogram or PE-Cy7 Mouse Anti-Human ICOS CD278 antibody Cat. No. 567395/567396; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing ICOS CD278 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of ICOS CD278 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either PE-Cy7 Mouse IgG1 kappa Isotype Control Cat. No. 565573; dashed line histogram or PE-Cy7 Mouse Anti-Human ICOS CD278 antibody Cat. No. 567395/567396; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing ICOS CD278 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of ICOS CD278 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either PE-Cy7 Mouse IgG1 kappa Isotype Control Cat. No. 565573; dashed line histogram or PE-Cy7 Mouse Anti-Human ICOS CD278 antibody Cat. No. 567395/567396; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing ICOS CD278 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.