BD PharmingenTM PE-Cy7 Rat Anti-Mouse CD103

Description

Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD103 antibody Cat. No. 567593; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD103 antibody Cat. No. 567593; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD103 antibody Cat. No. 567593; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD103 antibody Cat. No. 567593; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD103 antibody Cat. No. 567593; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD103 antibody Cat. No. 567593; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 or Ig Isotype Control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.