BD PharmingenTM PE-Cy7 Rat Anti-Mouse Ly-6C

Description

Multicolor flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 553030; Top Plots and BD Horizon BUV737 Rat Anti-CD11b antibody Cat. No. 612800; Bottom Plots and with either PE-Cy7 Rat IgG1, kappa Isotype Control Cat. No. 557645; Left Plots or PE-Cy7 Rat Anti-Mouse Ly-6C antibody Cat. No. 569442/569443; Right Plots at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a or CD11b were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific Multicolor flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 553030; Top Plots and BD Horizon BUV737 Rat Anti-CD11b antibody Cat. No. 612800; Bottom Plots and with either PE-Cy7 Rat IgG1, kappa Isotype Control Cat. No. 557645; Left Plots or PE-Cy7 Rat Anti-Mouse Ly-6C antibody Cat. No. 569442/569443; Right Plots at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a or CD11b were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific Show More Multicolor flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with FITC Rat Anti-Mouse CD8a antibody Cat. No. 553030; Top Plots and BD Horizon BUV737 Rat Anti-CD11b antibody Cat. No. 612800; Bottom Plots and with either PE-Cy7 Rat IgG1, kappa Isotype Control Cat. No. 557645; Left Plots or PE-Cy7 Rat Anti-Mouse Ly-6C antibody Cat. No. 569442/569443; Right Plots at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a or CD11b were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific