BD PharmingenTM PE-CyTM7 Hamster Anti-Mouse CD69

Description

Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours at 37C with Phorbol 12-Myristate 13-Acetate PMA, 10 ng/mL; Sigma-Aldrich, Cat. No. P-8139 . The cells were then stained with either PE-Cy7 Hamster IgG1, lambda1 Isotype Control Cat. No. 557798; dashed line histogram or PE-Cy7 Hamster Anti-Mouse CD69 antibody Cat. No. 552879/561930; solid line histogram at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours at 37C with Phorbol 12-Myristate 13-Acetate PMA, 10 ng/mL; Sigma-Aldrich, Cat. No. P-8139 . The cells were then stained with either PE-Cy7 Hamster IgG1, lambda1 Isotype Control Cat. No. 557798; dashed line histogram or PE-Cy7 Hamster Anti-Mouse CD69 antibody Cat. No. 552879/561930; solid line histogram at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours at 37C with Phorbol 12-Myristate 13-Acetate PMA, 10 ng/mL; Sigma-Aldrich, Cat. No. P-8139 . The cells were then stained with either PE-Cy7 Hamster IgG1, lambda1 Isotype Control Cat. No. 557798; dashed line histogram or PE-Cy7 Hamster Anti-Mouse CD69 antibody Cat. No. 552879/561930; solid line histogram at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.