BD PharmingenTM PE-CyTM7 Mouse Anti-Human MIP-1beta

Description

Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized, and stained with either a PE-Cy7 Mouse IgG1, kappa isotype control shaded or with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized, and stained with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized, and stained with either a PE-Cy7 Mouse IgG1, kappa isotype control shaded or with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized, and stained with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized, and stained with either a PE-Cy7 Mouse IgG1, kappa isotype control shaded or with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized, and stained with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized, and stained with either a PE-Cy7 Mouse IgG1, kappa isotype control shaded or with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized, and stained with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized, and stained with either a PE-Cy7 Mouse IgG1, kappa isotype control shaded or with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized, and stained with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized, and stained with either a PE-Cy7 Mouse IgG1, kappa isotype control shaded or with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized, and stained with the PE-Cy7 Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system.