BD PharmingenTM PE-CyTM7 Mouse Anti-Human TNF

Description

Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 4 hrs with PMA 5 ng/ml, Sigma and Ionomycin 500 ng, Sigma in the presence of Brefeldin A Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE mouse anti-human CD8 Cat. No. 555367 and either PE-Cy7 mouse anti-human TNF antibody Cat. No.557647/560923/560678, left panel or PE-Cy7 Mouse IgG1 kappa Isotype Control Cat. No. 557646, right panel . To demonstrate specificity of staining, the binding of PE-Cy7 Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF 0.25 mug,Cat. No. 554618 and the fixed/permeabilized cells with an excess of Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510 data not shown prior to staining. Quadrant markers were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 4 hrs with PMA 5 ng/ml, Sigma and Ionomycin 500 ng, Sigma in the presence of Brefeldin A Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE mouse anti-human CD8 Cat. No. 555367 and either PE-Cy7 mouse anti-human TNF antibody Cat. No.557647/560923/560678, left panel or PE-Cy7 Mouse IgG1 kappa Isotype Control Cat. No. 557646, right panel . To demonstrate specificity of staining, the binding of PE-Cy7 Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF 0.25 mug,Cat. No. 554618 and the fixed/permeabilized cells with an excess of Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510 data not shown prior to staining. Quadrant markers were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 4 hrs with PMA 5 ng/ml, Sigma and Ionomycin 500 ng, Sigma in the presence of Brefeldin A Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE mouse anti-human CD8 Cat. No. 555367 and either PE-Cy7 mouse anti-human TNF antibody Cat. No.557647/560923/560678, left panel or PE-Cy7 Mouse IgG1 kappa Isotype Control Cat. No. 557646, right panel . To demonstrate specificity of staining, the binding of PE-Cy7 Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF 0.25 mug,Cat. No. 554618 and the fixed/permeabilized cells with an excess of Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510 data not shown prior to staining. Quadrant markers were set based on the autofluorescence and isotype controls. Show More Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 4 hrs with PMA 5 ng/ml, Sigma and Ionomycin 500 ng, Sigma in the presence of Brefeldin A Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE mouse anti-human CD8 Cat. No. 555367 and either PE-Cy7 mouse anti-human TNF antibody Cat. No.557647/560923/560678, left panel or PE-Cy7 Mouse IgG1 kappa Isotype Control Cat. No. 557646, right panel . To demonstrate specificity of staining, the binding of PE-Cy7 Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF 0.25 mug,Cat. No. 554618 and the fixed/permeabilized cells with an excess of Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510 data not shown prior to staining. Quadrant markers were set based on the autofluorescence and isotype controls. Show More Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 4 hrs with PMA 5 ng/ml, Sigma and Ionomycin 500 ng, Sigma in the presence of Brefeldin A Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE mouse anti-human CD8 Cat. No. 555367 and either PE-Cy7 mouse anti-human TNF antibody Cat. No.557647/560923/560678, left panel or PE-Cy7 Mouse IgG1 kappa Isotype Control Cat. No. 557646, right panel . To demonstrate specificity of staining, the binding of PE-Cy7 Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF 0.25 mug,Cat. No. 554618 and the fixed/permeabilized cells with an excess of Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510 data not shown prior to staining. Quadrant markers were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 4 hrs with PMA 5 ng/ml, Sigma and Ionomycin 500 ng, Sigma in the presence of Brefeldin A Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE mouse anti-human CD8 Cat. No. 555367 and either PE-Cy7 mouse anti-human TNF antibody Cat. No.557647/560923/560678, left panel or PE-Cy7 Mouse IgG1 kappa Isotype Control Cat. No. 557646, right panel . To demonstrate specificity of staining, the binding of PE-Cy7 Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF 0.25 mug,Cat. No. 554618 and the fixed/permeabilized cells with an excess of Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510 data not shown prior to staining. Quadrant markers were set based on the autofluorescence and isotype controls.