BD PharmingenTM PE-CyTM7 Rat Anti-Mouse CD19

Description

Two-color analysis of the expression of CD19 on mouse spleen B cells. C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061 and either PE-Cy7-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 552784, left panel or PE-Cy7-conjugated 1D3 mAb right panel , in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4 G2 Cat. No. 553141 . Please note that the nonviable leukocytes were not exluded in this experiment, and the typical diagonal dead-cell population appears in the left panel. The same dead-cell population is partially obscured in the right panel. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Two-color analysis of the expression of CD19 on mouse spleen B cells. C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061 and either PE-Cy7-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 552784, left panel or PE-Cy7-conjugated 1D3 mAb right panel , in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4 G2 Cat. No. 553141 . Please note that the nonviable leukocytes were not exluded in this experiment, and the typical diagonal dead-cell population appears in the left panel. The same dead-cell population is partially obscured in the right panel. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Two-color analysis of the expression of CD19 on mouse spleen B cells. C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061 and either PE-Cy7-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 552784, left panel or PE-Cy7-conjugated 1D3 mAb right panel , in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4 G2 Cat. No. 553141 . Please note that the nonviable leukocytes were not exluded in this experiment, and the typical diagonal dead-cell population appears in the left panel. The same dead-cell population is partially obscured in the right panel. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Two-color analysis of the expression of CD19 on mouse spleen B cells. C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061 and either PE-Cy7-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 552784, left panel or PE-Cy7-conjugated 1D3 mAb right panel , in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4 G2 Cat. No. 553141 . Please note that the nonviable leukocytes were not exluded in this experiment, and the typical diagonal dead-cell population appears in the left panel. The same dead-cell population is partially obscured in the right panel. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Two-color analysis of the expression of CD19 on mouse spleen B cells. C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061 and either PE-Cy7-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 552784, left panel or PE-Cy7-conjugated 1D3 mAb right panel , in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4 G2 Cat. No. 553141 . Please note that the nonviable leukocytes were not exluded in this experiment, and the typical diagonal dead-cell population appears in the left panel. The same dead-cell population is partially obscured in the right panel. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Two-color analysis of the expression of CD19 on mouse spleen B cells. C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553061 and either PE-Cy7-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 552784, left panel or PE-Cy7-conjugated 1D3 mAb right panel , in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4 G2 Cat. No. 553141 . Please note that the nonviable leukocytes were not exluded in this experiment, and the typical diagonal dead-cell population appears in the left panel. The same dead-cell population is partially obscured in the right panel. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.