
558140 ·
BDB Bioscience · Cat: 558140
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD357 GITR expression on mouse CD4-positive splenocytes. C57BL/6 splenocytes were stained with APC Rat anti-Mouse CD25 Cat. No. 557192 , FITC Rat anti-Mouse CD4 Cat. No. 553046/553047 , and either PE-Cy7 Rat IgG2b Isotype Control Cat. No. 552849; open histograms or PE-Cy7 Rat anti-Mouse CD357 GITR Cat. No. 558139; shaded histograms . Viable leukocytes were selected by exclusion of propidium iodide Cat. No. 556463 . The left panel represents the expression of CD357 GITR by CD25-positive/CD4-positive T lymphocytes Treg cells , and the right panel shows CD25-negative/CD4-positive T lymphocytes. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Flow cytometric analysis of CD357 GITR expression on mouse CD4-positive splenocytes. C57BL/6 splenocytes were stained with APC Rat anti-Mouse CD25 Cat. No. 557192 , FITC Rat anti-Mouse CD4 Cat. No. 553046/553047 , and either PE-Cy7 Rat IgG2b Isotype Control Cat. No. 552849; open histograms or PE-Cy7 Rat anti-Mouse CD357 GITR Cat. No. 558139; shaded histograms . Viable leukocytes were selected by exclusion of propidium iodide Cat. No. 556463 . The left panel represents the expression of CD357 GITR by CD25-positive/CD4-positive T lymphocytes Treg cells , and the right panel shows CD25-negative/CD4-positive T lymphocytes. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Flow cytometric analysis of CD357 GITR expression on mouse CD4-positive splenocytes. C57BL/6 splenocytes were stained with APC Rat anti-Mouse CD25 Cat. No. 557192 , FITC Rat anti-Mouse CD4 Cat. No. 553046/553047 , and either PE-Cy7 Rat IgG2b Isotype Control Cat. No. 552849; open histograms or PE-Cy7 Rat anti-Mouse CD357 GITR Cat. No. 558139; shaded histograms . Viable leukocytes were selected by exclusion of propidium iodide Cat. No. 556463 . The left panel represents the expression of CD357 GITR by CD25-positive/CD4-positive T lymphocytes Treg cells , and the right panel shows CD25-negative/CD4-positive T lymphocytes. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.