BD PharmingenTM PE-CyTM7 Rat Anti-Mouse CD45

Description

Flow cytometric analysis of CD45 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either PE-Cy7 Rat IgG2b, kappa Isotype Control Cat. No. 552849; dashed line histogram or PE-Cy7 Rat Anti-Mouse CD45 antibody Cat. No. 552848/561868; solid line histogram at 0.06 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45 fluorescence or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis was performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either PE-Cy7 Rat IgG2b, kappa Isotype Control Cat. No. 552849; dashed line histogram or PE-Cy7 Rat Anti-Mouse CD45 antibody Cat. No. 552848/561868; solid line histogram at 0.06 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45 fluorescence or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis was performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45 expression on mouse splenocytes. Mouse splenic leucocytes were stained with either PE-Cy7 Rat IgG2b, kappa Isotype Control Cat. No. 552849; dashed line histogram or PE-Cy7 Rat Anti-Mouse CD45 antibody Cat. No. 552848/561868; solid line histogram at 0.06 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45 fluorescence or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis was performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.