BD PharmingenTM PE-CyTM7 Rat Anti-Mouse CD8a

Description

Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD8a Cat. No. 552877/561097; Right Plot antibodies at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot-specific. Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD8a Cat. No. 552877/561097; Right Plot antibodies at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot-specific. Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD8a Cat. No. 552877/561097; Right Plot antibodies at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot-specific. Show More Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD8a Cat. No. 552877/561097; Right Plot antibodies at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot-specific. Show More Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD8a Cat. No. 552877/561097; Right Plot antibodies at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot-specific. Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with FITC Rat Anti-Mouse CD3e Cat. No. 553061/553062/561827 and either PE-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552784; Left Plot or PE-Cy7 Rat Anti-Mouse CD8a Cat. No. 552877/561097; Right Plot antibodies at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot-specific.