BD PharmingenTM PE-CyTM7 Rat IgG1 kappa Isotype Control

Description

Expression of TNF by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with APC-conjugated rat anti-mouse CD4 APC-RM4-5, Cat. No. 553051 and either rat anti-mouse TNF antibody PE-Cy7-MP6-XT22, Cat. No. 557644 left panel or immunoglobulin isotype control PE-Cy7-R3-34, Cat. No. 557645 , right panel by using Pharmingens staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with APC-conjugated rat anti-mouse CD4 APC-RM4-5, Cat. No. 553051 and either rat anti-mouse TNF antibody PE-Cy7-MP6-XT22, Cat. No. 557644 left panel or immunoglobulin isotype control PE-Cy7-R3-34, Cat. No. 557645 , right panel by using Pharmingens staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with APC-conjugated rat anti-mouse CD4 APC-RM4-5, Cat. No. 553051 and either rat anti-mouse TNF antibody PE-Cy7-MP6-XT22, Cat. No. 557644 left panel or immunoglobulin isotype control PE-Cy7-R3-34, Cat. No. 557645 , right panel by using Pharmingens staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Show More Expression of TNF by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with APC-conjugated rat anti-mouse CD4 APC-RM4-5, Cat. No. 553051 and either rat anti-mouse TNF antibody PE-Cy7-MP6-XT22, Cat. No. 557644 left panel or immunoglobulin isotype control PE-Cy7-R3-34, Cat. No. 557645 , right panel by using Pharmingens staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Show More Expression of TNF by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with APC-conjugated rat anti-mouse CD4 APC-RM4-5, Cat. No. 553051 and either rat anti-mouse TNF antibody PE-Cy7-MP6-XT22, Cat. No. 557644 left panel or immunoglobulin isotype control PE-Cy7-R3-34, Cat. No. 557645 , right panel by using Pharmingens staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with APC-conjugated rat anti-mouse CD4 APC-RM4-5, Cat. No. 553051 and either rat anti-mouse TNF antibody PE-Cy7-MP6-XT22, Cat. No. 557644 left panel or immunoglobulin isotype control PE-Cy7-R3-34, Cat. No. 557645 , right panel by using Pharmingens staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls.