BD PharmingenTM PE Hamster Anti-Mouse CD103

Description

Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Lymph node cells from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either PE Hamster IgG2, kappa Isotype Control Cat. No. 550085; Left Plot or PE Rat Anti-Mouse CD103 antibody Cat. No. 566844; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD103 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-nrgative leucocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Lymph node cells from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either PE Hamster IgG2, kappa Isotype Control Cat. No. 550085; Left Plot or PE Rat Anti-Mouse CD103 antibody Cat. No. 566844; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD103 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-nrgative leucocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Lymph node cells from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either PE Hamster IgG2, kappa Isotype Control Cat. No. 550085; Left Plot or PE Rat Anti-Mouse CD103 antibody Cat. No. 566844; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD103 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-nrgative leucocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Lymph node cells from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either PE Hamster IgG2, kappa Isotype Control Cat. No. 550085; Left Plot or PE Rat Anti-Mouse CD103 antibody Cat. No. 566844; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD103 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-nrgative leucocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Lymph node cells from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either PE Hamster IgG2, kappa Isotype Control Cat. No. 550085; Left Plot or PE Rat Anti-Mouse CD103 antibody Cat. No. 566844; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD103 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-nrgative leucocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD103 expression on mouse lymph node cells. Lymph node cells from a C57BL/6 mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either PE Hamster IgG2, kappa Isotype Control Cat. No. 550085; Left Plot or PE Rat Anti-Mouse CD103 antibody Cat. No. 566844; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD103 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-nrgative leucocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.