BD PharmingenTM PE Hamster Anti-Mouse CD137 (4-1BB)

Description

Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated with Concanavalin A Con A for three days and then stained with APC Rat Anti-Mouse CD25 antibody Cat. No. 558643 and with either PE Hamster IgG2, lambda1 Isotype Control Cat. No. 553965; Left Plot or PE Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 569975/569976; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or Ig Isotype control staining versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated with Concanavalin A Con A for three days and then stained with APC Rat Anti-Mouse CD25 antibody Cat. No. 558643 and with either PE Hamster IgG2, lambda1 Isotype Control Cat. No. 553965; Left Plot or PE Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 569975/569976; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or Ig Isotype control staining versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated with Concanavalin A Con A for three days and then stained with APC Rat Anti-Mouse CD25 antibody Cat. No. 558643 and with either PE Hamster IgG2, lambda1 Isotype Control Cat. No. 553965; Left Plot or PE Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 569975/569976; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or Ig Isotype control staining versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated with Concanavalin A Con A for three days and then stained with APC Rat Anti-Mouse CD25 antibody Cat. No. 558643 and with either PE Hamster IgG2, lambda1 Isotype Control Cat. No. 553965; Left Plot or PE Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 569975/569976; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or Ig Isotype control staining versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated with Concanavalin A Con A for three days and then stained with APC Rat Anti-Mouse CD25 antibody Cat. No. 558643 and with either PE Hamster IgG2, lambda1 Isotype Control Cat. No. 553965; Left Plot or PE Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 569975/569976; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or Ig Isotype control staining versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leucocytes. BALB/c Mouse splenocytes were stimulated with Concanavalin A Con A for three days and then stained with APC Rat Anti-Mouse CD25 antibody Cat. No. 558643 and with either PE Hamster IgG2, lambda1 Isotype Control Cat. No. 553965; Left Plot or PE Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 569975/569976; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or Ig Isotype control staining versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.