BD PharmingenTM PE Hamster Anti-Mouse CD209b (SIGN-R1)

Description

Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on BALB/c or C57BL/6 Mouse resident peritoneal cells. Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either PE Hamster Isotype Control Cat. No. 550085, Left Plots or PE Armenian Hamster Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 568962/568963, Right Plots at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD209b SIGN-R1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative resident peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on BALB/c or C57BL/6 Mouse resident peritoneal cells. Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either PE Hamster Isotype Control Cat. No. 550085, Left Plots or PE Armenian Hamster Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 568962/568963, Right Plots at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD209b SIGN-R1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative resident peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on BALB/c or C57BL/6 Mouse resident peritoneal cells. Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either PE Hamster Isotype Control Cat. No. 550085, Left Plots or PE Armenian Hamster Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 568962/568963, Right Plots at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD209b SIGN-R1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative resident peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.