BD PharmingenTM PE Hamster Anti-Mouse CD54

Description

Upregulation of CD54 expression on activated splenic B lymphocytes. Left panel : Naive BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable resting lymphocytes were gated according to scatter profile and exclusion of 7-AAD BD Via-Probe, Cat. No. 555816/555815 . The mean fluorescence intensity of the stained lymphocytes is about 50 times greater than that of the negative-control lymphocytes. Right panel: 2-day LPS-activated BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable B-cell blasts were gated according to scatter profile and exclusion of 7-AAD. The mean fluorescence intensity of the stained blasts is about 180 times greater than that of the negative-control blasts. Flow cytometry was performed on a BD FACScan flow cytometry system. Upregulation of CD54 expression on activated splenic B lymphocytes. Left panel : Naive BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable resting lymphocytes were gated according to scatter profile and exclusion of 7-AAD BD Via-Probe, Cat. No. 555816/555815 . The mean fluorescence intensity of the stained lymphocytes is about 50 times greater than that of the negative-control lymphocytes. Right panel: 2-day LPS-activated BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable B-cell blasts were gated according to scatter profile and exclusion of 7-AAD. The mean fluorescence intensity of the stained blasts is about 180 times greater than that of the negative-control blasts. Flow cytometry was performed on a BD FACScan flow cytometry system. Upregulation of CD54 expression on activated splenic B lymphocytes. Left panel : Naive BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable resting lymphocytes were gated according to scatter profile and exclusion of 7-AAD BD Via-Probe, Cat. No. 555816/555815 . The mean fluorescence intensity of the stained lymphocytes is about 50 times greater than that of the negative-control lymphocytes. Right panel: 2-day LPS-activated BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable B-cell blasts were gated according to scatter profile and exclusion of 7-AAD. The mean fluorescence intensity of the stained blasts is about 180 times greater than that of the negative-control blasts. Flow cytometry was performed on a BD FACScan flow cytometry system. Show More Upregulation of CD54 expression on activated splenic B lymphocytes. Left panel : Naive BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable resting lymphocytes were gated according to scatter profile and exclusion of 7-AAD BD Via-Probe, Cat. No. 555816/555815 . The mean fluorescence intensity of the stained lymphocytes is about 50 times greater than that of the negative-control lymphocytes. Right panel: 2-day LPS-activated BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable B-cell blasts were gated according to scatter profile and exclusion of 7-AAD. The mean fluorescence intensity of the stained blasts is about 180 times greater than that of the negative-control blasts. Flow cytometry was performed on a BD FACScan flow cytometry system. Show More Upregulation of CD54 expression on activated splenic B lymphocytes. Left panel : Naive BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable resting lymphocytes were gated according to scatter profile and exclusion of 7-AAD BD Via-Probe, Cat. No. 555816/555815 . The mean fluorescence intensity of the stained lymphocytes is about 50 times greater than that of the negative-control lymphocytes. Right panel: 2-day LPS-activated BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable B-cell blasts were gated according to scatter profile and exclusion of 7-AAD. The mean fluorescence intensity of the stained blasts is about 180 times greater than that of the negative-control blasts. Flow cytometry was performed on a BD FACScan flow cytometry system. Upregulation of CD54 expression on activated splenic B lymphocytes. Left panel : Naive BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable resting lymphocytes were gated according to scatter profile and exclusion of 7-AAD BD Via-Probe, Cat. No. 555816/555815 . The mean fluorescence intensity of the stained lymphocytes is about 50 times greater than that of the negative-control lymphocytes. Right panel: 2-day LPS-activated BALB/c splenocytes were stained with PE-conjugated 3E2 mAb open histogram or unstained filled histogram . Viable B-cell blasts were gated according to scatter profile and exclusion of 7-AAD. The mean fluorescence intensity of the stained blasts is about 180 times greater than that of the negative-control blasts. Flow cytometry was performed on a BD FACScan flow cytometry system.