BD PharmingenTM PE Hamster anti-Mouse CD55

Description

Two-color analysis of CD55 expression on splenic B lymphocytes. C57BL/6 splenocytes were pre-incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 Cat. No. 553141/553142 , stained with FITC anti-mouse CD45R/B220 Cat. No. 553087/553088 and either PE Hamster IgG3, lambda isotype control Cat. No. 553980, left panel or PE Hamster anti-Mouse CD55 Cat. No. 58037, right panel . Dead cells were excluded by staining with propidium iodide Cat. No. 556463 , and the total viable leukocytes are displayed. The data demonstrates that CD55 is expressed on the majority of splenocytes, with the greatest density on B lymphocytes. Flow cytometry was performed on a BD FACScalibur flow cytometry system. Two-color analysis of CD55 expression on splenic B lymphocytes. C57BL/6 splenocytes were pre-incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 Cat. No. 553141/553142 , stained with FITC anti-mouse CD45R/B220 Cat. No. 553087/553088 and either PE Hamster IgG3, lambda isotype control Cat. No. 553980, left panel or PE Hamster anti-Mouse CD55 Cat. No. 58037, right panel . Dead cells were excluded by staining with propidium iodide Cat. No. 556463 , and the total viable leukocytes are displayed. The data demonstrates that CD55 is expressed on the majority of splenocytes, with the greatest density on B lymphocytes. Flow cytometry was performed on a BD FACScalibur flow cytometry system. Two-color analysis of CD55 expression on splenic B lymphocytes. C57BL/6 splenocytes were pre-incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 Cat. No. 553141/553142 , stained with FITC anti-mouse CD45R/B220 Cat. No. 553087/553088 and either PE Hamster IgG3, lambda isotype control Cat. No. 553980, left panel or PE Hamster anti-Mouse CD55 Cat. No. 58037, right panel . Dead cells were excluded by staining with propidium iodide Cat. No. 556463 , and the total viable leukocytes are displayed. The data demonstrates that CD55 is expressed on the majority of splenocytes, with the greatest density on B lymphocytes. Flow cytometry was performed on a BD FACScalibur flow cytometry system. Show More Two-color analysis of CD55 expression on splenic B lymphocytes. C57BL/6 splenocytes were pre-incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 Cat. No. 553141/553142 , stained with FITC anti-mouse CD45R/B220 Cat. No. 553087/553088 and either PE Hamster IgG3, lambda isotype control Cat. No. 553980, left panel or PE Hamster anti-Mouse CD55 Cat. No. 58037, right panel . Dead cells were excluded by staining with propidium iodide Cat. No. 556463 , and the total viable leukocytes are displayed. The data demonstrates that CD55 is expressed on the majority of splenocytes, with the greatest density on B lymphocytes. Flow cytometry was performed on a BD FACScalibur flow cytometry system. Show More Two-color analysis of CD55 expression on splenic B lymphocytes. C57BL/6 splenocytes were pre-incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 Cat. No. 553141/553142 , stained with FITC anti-mouse CD45R/B220 Cat. No. 553087/553088 and either PE Hamster IgG3, lambda isotype control Cat. No. 553980, left panel or PE Hamster anti-Mouse CD55 Cat. No. 58037, right panel . Dead cells were excluded by staining with propidium iodide Cat. No. 556463 , and the total viable leukocytes are displayed. The data demonstrates that CD55 is expressed on the majority of splenocytes, with the greatest density on B lymphocytes. Flow cytometry was performed on a BD FACScalibur flow cytometry system. Two-color analysis of CD55 expression on splenic B lymphocytes. C57BL/6 splenocytes were pre-incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 Cat. No. 553141/553142 , stained with FITC anti-mouse CD45R/B220 Cat. No. 553087/553088 and either PE Hamster IgG3, lambda isotype control Cat. No. 553980, left panel or PE Hamster anti-Mouse CD55 Cat. No. 58037, right panel . Dead cells were excluded by staining with propidium iodide Cat. No. 556463 , and the total viable leukocytes are displayed. The data demonstrates that CD55 is expressed on the majority of splenocytes, with the greatest density on B lymphocytes. Flow cytometry was performed on a BD FACScalibur flow cytometry system.