BD PharmingenTM PE Hamster Anti-Mouse CD69

Description

Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours at 37C with Phorbol 12-Myristate 13-Acetate PMA, 10 ng/mL; Sigma-Aldrich, Cat. No. P-8139 . The stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either PE Hamster IgG1, lambda1 Isotype Control Cat. No. 553954; dashed line histogram or PE Hamster Anti-Mouse CD69 antibody Cat. No. 561932/553237; solid line histogram at 0.06 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative stimulated leucocytes. Flow cytometry and data analysis was performed using a BD FACSCelesta Flow Cytometer System and FloJo software. Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours at 37C with Phorbol 12-Myristate 13-Acetate PMA, 10 ng/mL; Sigma-Aldrich, Cat. No. P-8139 . The stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either PE Hamster IgG1, lambda1 Isotype Control Cat. No. 553954; dashed line histogram or PE Hamster Anti-Mouse CD69 antibody Cat. No. 561932/553237; solid line histogram at 0.06 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative stimulated leucocytes. Flow cytometry and data analysis was performed using a BD FACSCelesta Flow Cytometer System and FloJo software. Show More Flow cytometric analysis of CD69 expression on stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours at 37C with Phorbol 12-Myristate 13-Acetate PMA, 10 ng/mL; Sigma-Aldrich, Cat. No. P-8139 . The stimulated cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either PE Hamster IgG1, lambda1 Isotype Control Cat. No. 553954; dashed line histogram or PE Hamster Anti-Mouse CD69 antibody Cat. No. 561932/553237; solid line histogram at 0.06 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative stimulated leucocytes. Flow cytometry and data analysis was performed using a BD FACSCelesta Flow Cytometer System and FloJo software.