BD PharmingenTM PE Hamster Anti-Mouse CXCL9 (MIG)

Description

Flow cytometric analysis of CXCL9 MIG expression in Stimulated mouse peritoneal exudate cells PEC . Thioglycolate-elicited BALB/c mouse PEC were cultured in complete tissue culture medium with Recombinant Mouse IFN-gamma protein Cat. No. 554587, 10 ng/ml for 2 hours followed by the addition of lipopolysaccharide LPS; Sigma, 1 mug/ml . BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 was then added to both cultures that were further cultured overnight. The cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with Alexa Fluor 647 Rat Anti-Mouse CD11b Cat. No. 557686 and either PE Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or PE Armenian Hamster Anti-Mouse CXCL9 MIG antibody Cat. No. 567163; Left Plot at 0.25 mug/test. The two-color flow cytometric contour plots showing CXCL9 MIG expression or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CXCL9 MIG expression in Unstimulated and Stimulated RAW264.7 cells . Cells from the mouse RAW 264.7 Macrophage; ATCC TIB-71 cell line were cultured in complete tissue culture medium without activators Unstimulated Cells or with Recombinant Mouse IFN-gamma protein Cat. No. 554587, 10 ng/ml for 2 hours followed by the addition of lipopolysaccharide LPS; Sigma, 1 mug/ml Stimulated Cells . BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 was then added to both cultures that were further cultured overnight. The cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with PE Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553972; dashed line histogram or PE Armenian Hamster Anti-CXCL9 MIG antibody Cat. No. 567163; solid and dotted line histograms at 0.25 mug/test. Left Plot: The fluorescence histogram showing CXCL9 MIG expression solid line or Ig Isotype control staining dashed line were derived from gated events with the light-scatter characteristics of intact cells. Right Plot: The histograms showing CXCL9 MIG expression in Unstimulated Cells dotted line versus Stimulated Cells solid line were overlaid for the sake of direct comparison as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CXCL9 MIG expression in Stimulated mouse peritoneal exudate cells PEC . Thioglycolate-elicited BALB/c mouse PEC were cultured in complete tissue culture medium with Recombinant Mouse IFN-gamma protein Cat. No. 554587, 10 ng/ml for 2 hours followed by the addition of lipopolysaccharide LPS; Sigma, 1 mug/ml . BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 was then added to both cultures that were further cultured overnight. The cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with Alexa Fluor 647 Rat Anti-Mouse CD11b Cat. No. 557686 and either PE Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or PE Armenian Hamster Anti-Mouse CXCL9 MIG antibody Cat. No. 567163; Left Plot at 0.25 mug/test. The two-color flow cytometric contour plots showing CXCL9 MIG expression or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CXCL9 MIG expression in Unstimulated and Stimulated RAW264.7 cells . Cells from the mouse RAW 264.7 Macrophage; ATCC TIB-71 cell line were cultured in complete tissue culture medium without activators Unstimulated Cells or with Recombinant Mouse IFN-gamma protein Cat. No. 554587, 10 ng/ml for 2 hours followed by the addition of lipopolysaccharide LPS; Sigma, 1 mug/ml Stimulated Cells . BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 was then added to both cultures that were further cultured overnight. The cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with PE Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553972; dashed line histogram or PE Armenian Hamster Anti-CXCL9 MIG antibody Cat. No. 567163; solid and dotted line histograms at 0.25 mug/test. Left Plot: The fluorescence histogram showing CXCL9 MIG expression solid line or Ig Isotype control staining dashed line were derived from gated events with the light-scatter characteristics of intact cells. Right Plot: The histograms showing CXCL9 MIG expression in Unstimulated Cells dotted line versus Stimulated Cells solid line were overlaid for the sake of direct comparison as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CXCL9 MIG expression in Stimulated mouse peritoneal exudate cells PEC . Thioglycolate-elicited BALB/c mouse PEC were cultured in complete tissue culture medium with Recombinant Mouse IFN-gamma protein Cat. No. 554587, 10 ng/ml for 2 hours followed by the addition of lipopolysaccharide LPS; Sigma, 1 mug/ml . BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 was then added to both cultures that were further cultured overnight. The cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with Alexa Fluor 647 Rat Anti-Mouse CD11b Cat. No. 557686 and either PE Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or PE Armenian Hamster Anti-Mouse CXCL9 MIG antibody Cat. No. 567163; Left Plot at 0.25 mug/test. The two-color flow cytometric contour plots showing CXCL9 MIG expression or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CXCL9 MIG expression in Unstimulated and Stimulated RAW264.7 cells . Cells from the mouse RAW 264.7 Macrophage; ATCC TIB-71 cell line were cultured in complete tissue culture medium without activators Unstimulated Cells or with Recombinant Mouse IFN-gamma protein Cat. No. 554587, 10 ng/ml for 2 hours followed by the addition of lipopolysaccharide LPS; Sigma, 1 mug/ml Stimulated Cells . BD GolgiPlug Protein Transport Inhibitor Cat. No. 555029 was then added to both cultures that were further cultured overnight. The cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with PE Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553972; dashed line histogram or PE Armenian Hamster Anti-CXCL9 MIG antibody Cat. No. 567163; solid and dotted line histograms at 0.25 mug/test. Left Plot: The fluorescence histogram showing CXCL9 MIG expression solid line or Ig Isotype control staining dashed line were derived from gated events with the light-scatter characteristics of intact cells. Right Plot: The histograms showing CXCL9 MIG expression in Unstimulated Cells dotted line versus Stimulated Cells solid line were overlaid for the sake of direct comparison as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.