BD PharmingenTM PE Hamster Anti-Mouse FcepsilonR1alpha

Description

Flow cytometric analysis of FcepsilonRIalpha expression on MC/9 cells. Cells from the mouse MC/9 Mast Cells, ATCC CRL-8306 cell line were stained with PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; dashed line histogram or PE Mouse Anti-FcepsilonRIalpha antibody Cat. No. 566994/566995; solid line histogram at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The fluorescence histogram showing FcepsilonRIalpha expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of FcepsilonRIalpha expression on mouse spleen cells. C57BL/6 mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , FITC Rat anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 , APC Rat Anti-Mouse CD49b Cat. No. 560628 antibodies and either PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or PE Hamster Anti-Mouse FcepsilonRIalpha antibody Cat. No. 566994/566995; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Two-parameter pseudocolor density plots showing the correlated expression of FcepsilonRIalpha or Ig Isotype control staining versus CD49b were derived from CD3-negative B220-negative gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of FcepsilonRIalpha expression on MC/9 cells. Cells from the mouse MC/9 Mast Cells, ATCC CRL-8306 cell line were stained with PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; dashed line histogram or PE Mouse Anti-FcepsilonRIalpha antibody Cat. No. 566994/566995; solid line histogram at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The fluorescence histogram showing FcepsilonRIalpha expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of FcepsilonRIalpha expression on mouse spleen cells. C57BL/6 mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , FITC Rat anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 , APC Rat Anti-Mouse CD49b Cat. No. 560628 antibodies and either PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or PE Hamster Anti-Mouse FcepsilonRIalpha antibody Cat. No. 566994/566995; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Two-parameter pseudocolor density plots showing the correlated expression of FcepsilonRIalpha or Ig Isotype control staining versus CD49b were derived from CD3-negative B220-negative gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of FcepsilonRIalpha expression on MC/9 cells. Cells from the mouse MC/9 Mast Cells, ATCC CRL-8306 cell line were stained with PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; dashed line histogram or PE Mouse Anti-FcepsilonRIalpha antibody Cat. No. 566994/566995; solid line histogram at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The fluorescence histogram showing FcepsilonRIalpha expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of FcepsilonRIalpha expression on mouse spleen cells. C57BL/6 mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , FITC Rat anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 , APC Rat Anti-Mouse CD49b Cat. No. 560628 antibodies and either PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or PE Hamster Anti-Mouse FcepsilonRIalpha antibody Cat. No. 566994/566995; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Two-parameter pseudocolor density plots showing the correlated expression of FcepsilonRIalpha or Ig Isotype control staining versus CD49b were derived from CD3-negative B220-negative gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.