BD PharmingenTM PE Hamster Anti-Mouse Notch4

Description

Two-color flow cytometric analysis of Notch4 expression on Mouse splenic dendritic cells. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c Cat. No. 550261 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No.563786 antibodies and with either PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or BD Pharmingen PE Hamster Anti-Mouse Notch4 antibody Cat. No. 568785; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Notch4 expression on Mouse splenic dendritic cells. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c Cat. No. 550261 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No.563786 antibodies and with either PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or BD Pharmingen PE Hamster Anti-Mouse Notch4 antibody Cat. No. 568785; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Notch4 expression on Mouse splenic dendritic cells. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c Cat. No. 550261 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No.563786 antibodies and with either PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or BD Pharmingen PE Hamster Anti-Mouse Notch4 antibody Cat. No. 568785; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Notch4 expression on Mouse splenic dendritic cells. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c Cat. No. 550261 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No.563786 antibodies and with either PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or BD Pharmingen PE Hamster Anti-Mouse Notch4 antibody Cat. No. 568785; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Notch4 expression on Mouse splenic dendritic cells. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c Cat. No. 550261 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No.563786 antibodies and with either PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or BD Pharmingen PE Hamster Anti-Mouse Notch4 antibody Cat. No. 568785; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Notch4 expression on Mouse splenic dendritic cells. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD11c Cat. No. 550261 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No.563786 antibodies and with either PE Hamster IgG1, kappa Isotype Control Cat. No. 553972; Left Plot or BD Pharmingen PE Hamster Anti-Mouse Notch4 antibody Cat. No. 568785; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Notch4 or Ig Isotype control staining versus CD8a was derived from gated CD11c+ events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.