

565711 ·
BDB Bioscience · Cat: 565711
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multiparameter flow cytometric analysis of AhR expression Top Plots - AhR expression in human peripheral blood mononuclear cells. PBMCs were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-AhR antibody Cat. No. 565711; Right Plot . Bottom Plots - AhR expression in mouse splenic leucocytes. Mouse splenic leucocytes were similarly fixed, permeabilized, stained, and analyzed. Two-parameter flow cytometric contour plots showing the correlated expression of AhR or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Multiparameter flow cytometric analysis of AhR expression Top Plots - AhR expression in human peripheral blood mononuclear cells. PBMCs were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-AhR antibody Cat. No. 565711; Right Plot . Bottom Plots - AhR expression in mouse splenic leucocytes. Mouse splenic leucocytes were similarly fixed, permeabilized, stained, and analyzed. Two-parameter flow cytometric contour plots showing the correlated expression of AhR or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Multiparameter flow cytometric analysis of AhR expression Top Plots - AhR expression in human peripheral blood mononuclear cells. PBMCs were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 and stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-AhR antibody Cat. No. 565711; Right Plot . Bottom Plots - AhR expression in mouse splenic leucocytes. Mouse splenic leucocytes were similarly fixed, permeabilized, stained, and analyzed. Two-parameter flow cytometric contour plots showing the correlated expression of AhR or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System.