BD PharmingenTM PE Mouse anti-BATF

Description

Flow cytometric analysis of BATF expression in Human and Mouse Lymphocytes. Left Panel - Human peripheral blood mononuclear cells PBMC were cultured 3 days with immobilized Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml for plate coating and soluble Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 2 mug/ml antibodies. Freshly-prepared PBMC from the same donor dotted line histograms and the stimulated PBMC solid line histograms were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human BATF antibody Cat. No. 564503; Right Plot . Right Panel - Mouse splenic leucocytes were cultured 3 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies. Freshly-prepared splenic leucocytes dotted line histograms and the stimulated splenocytes solid line histograms were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set. The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-Human BATF antibody Right Plot . Fluorescence histograms showing the expression of BATF or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of BATF expression in Human and Mouse Lymphocytes. Left Panel - Human peripheral blood mononuclear cells PBMC were cultured 3 days with immobilized Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml for plate coating and soluble Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 2 mug/ml antibodies. Freshly-prepared PBMC from the same donor dotted line histograms and the stimulated PBMC solid line histograms were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human BATF antibody Cat. No. 564503; Right Plot . Right Panel - Mouse splenic leucocytes were cultured 3 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies. Freshly-prepared splenic leucocytes dotted line histograms and the stimulated splenocytes solid line histograms were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set. The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-Human BATF antibody Right Plot . Fluorescence histograms showing the expression of BATF or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of BATF expression in Human and Mouse Lymphocytes. Left Panel - Human peripheral blood mononuclear cells PBMC were cultured 3 days with immobilized Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml for plate coating and soluble Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 2 mug/ml antibodies. Freshly-prepared PBMC from the same donor dotted line histograms and the stimulated PBMC solid line histograms were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human BATF antibody Cat. No. 564503; Right Plot . Right Panel - Mouse splenic leucocytes were cultured 3 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies. Freshly-prepared splenic leucocytes dotted line histograms and the stimulated splenocytes solid line histograms were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set. The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Left Plot or PE Mouse Anti-Human BATF antibody Right Plot . Fluorescence histograms showing the expression of BATF or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.