BD PharmingenTM PE Mouse anti-Bcl-6

Description

Bcl-6 expression in Human B lymphocytes Left Panel . Tonsil cells were stained with BD Horizon V450 Anti-Human CD19 Cat. No. 560353 , PE-Cy7 Anti-Human CD4 Cat. No. 560649 and APC Anti-Human CD38 Cat. No. 555462 . Cells were washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by no staining Left Figure or intracellular staining with PE Mouse Anti-Bcl-6 Right Figure; Cat. No. 561522 . Contour plots show expression of Bcl-6 or background fluorescence versus CD38 by B cells identified as CD4-CD19+ events with the light-scatter characteristics of intact lymphocytes. Bcl-6 staining is observed in CD38+ germinal center B cells but not in CD38- naive B cells nor CD38++ plasma cells. Cytometry was performed with a BD FACSCanto II Flow Cytometer. Data courtesy of Mark Kroenke & Shane Crotty, La Jolla Institute for Allergy & Immunology. Bcl-6 expression in Mouse B lymphocytes Right Panel . BALB/c mouse mesenteric lymph node cells were stained with APC Anti-Mouse B220 Cat. No. 553092 , PerCP-Cy5.5 Anti-Mouse CD4 Cat. No. 550954 , FITC Anti-Mouse Fas/CD95 Cat. No. 554257 , and BD Horizon V450 Anti-Mouse IgD Cat. No. 560869 . Cells were washed, resuspended in RPMI containing 10 % FBS, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with PE Anti-Bcl-6 Cat. No. 561522 . A contour plot shows the expression of IgD versus Fas/CD95 by B cells identified as CD4-B220+ events with the light-scatter characteristics of intact lymphocytes Left Figure . Germinal center B cells were identified as IgDloFas+ B lymphocytes. Fluorescence histograms Right Figure show intracellular Bcl-6 staining of mouse germinal center B cells open histogram and non-GC B cells shaded histogram . Cytometry was performed with a BD LSRFortessa Flow Cytometer. Bcl-6 expression in Human B lymphocytes Left Panel . Tonsil cells were stained with BD Horizon V450 Anti-Human CD19 Cat. No. 560353 , PE-Cy7 Anti-Human CD4 Cat. No. 560649 and APC Anti-Human CD38 Cat. No. 555462 . Cells were washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by no staining Left Figure or intracellular staining with PE Mouse Anti-Bcl-6 Right Figure; Cat. No. 561522 . Contour plots show expression of Bcl-6 or background fluorescence versus CD38 by B cells identified as CD4-CD19+ events with the light-scatter characteristics of intact lymphocytes. Bcl-6 staining is observed in CD38+ germinal center B cells but not in CD38- naive B cells nor CD38++ plasma cells. Cytometry was performed with a BD FACSCanto II Flow Cytometer. Data courtesy of Mark Kroenke & Shane Crotty, La Jolla Institute for Allergy & Immunology. Bcl-6 expression in Mouse B lymphocytes Right Panel . BALB/c mouse mesenteric lymph node cells were stained with APC Anti-Mouse B220 Cat. No. 553092 , PerCP-Cy5.5 Anti-Mouse CD4 Cat. No. 550954 , FITC Anti-Mouse Fas/CD95 Cat. No. 554257 , and BD Horizon V450 Anti-Mouse IgD Cat. No. 560869 . Cells were washed, resuspended in RPMI containing 10 % FBS, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with PE Anti-Bcl-6 Cat. No. 561522 . A contour plot shows the expression of IgD versus Fas/CD95 by B cells identified as CD4-B220+ events with the light-scatter characteristics of intact lymphocytes Left Figure . Germinal center B cells were identified as IgDloFas+ B lymphocytes. Fluorescence histograms Right Figure show intracellular Bcl-6 staining of mouse germinal center B cells open histogram and non-GC B cells shaded histogram . Cytometry was performed with a BD LSRFortessa Flow Cytometer. Show More Bcl-6 expression in Human B lymphocytes Left Panel . Tonsil cells were stained with BD Horizon V450 Anti-Human CD19 Cat. No. 560353 , PE-Cy7 Anti-Human CD4 Cat. No. 560649 and APC Anti-Human CD38 Cat. No. 555462 . Cells were washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by no staining Left Figure or intracellular staining with PE Mouse Anti-Bcl-6 Right Figure; Cat. No. 561522 . Contour plots show expression of Bcl-6 or background fluorescence versus CD38 by B cells identified as CD4-CD19+ events with the light-scatter characteristics of intact lymphocytes. Bcl-6 staining is observed in CD38+ germinal center B cells but not in CD38- naive B cells nor CD38++ plasma cells. Cytometry was performed with a BD FACSCanto II Flow Cytometer. Data courtesy of Mark Kroenke & Shane Crotty, La Jolla Institute for Allergy & Immunology. Bcl-6 expression in Mouse B lymphocytes Right Panel . BALB/c mouse mesenteric lymph node cells were stained with APC Anti-Mouse B220 Cat. No. 553092 , PerCP-Cy5.5 Anti-Mouse CD4 Cat. No. 550954 , FITC Anti-Mouse Fas/CD95 Cat. No. 554257 , and BD Horizon V450 Anti-Mouse IgD Cat. No. 560869 . Cells were washed, resuspended in RPMI containing 10 % FBS, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with PE Anti-Bcl-6 Cat. No. 561522 . A contour plot shows the expression of IgD versus Fas/CD95 by B cells identified as CD4-B220+ events with the light-scatter characteristics of intact lymphocytes Left Figure . Germinal center B cells were identified as IgDloFas+ B lymphocytes. Fluorescence histograms Right Figure show intracellular Bcl-6 staining of mouse germinal center B cells open histogram and non-GC B cells shaded histogram . Cytometry was performed with a BD LSRFortessa Flow Cytometer.