BD PharmingenTM PE Mouse Anti- BrdU Set

Description

Two-color flow cytometric analysis of DNA synthesis by REH cells. REH Human leukemia, ATCC CRL-8286 cells were labeled with 1mM BrdU for one hour, washed, fixed with 75 % ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 kappa Isotype Control Left Panel or PE Mouse Anti-BrdU antibody Right Panel; Cat No. 556029 followed by staining with DAPI Solution Cat. No. 564907; nucleic acid stain . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System Two-color flow cytometric analysis of DNA synthesis by REH cells. REH Human leukemia, ATCC CRL-8286 cells were labeled with 1mM BrdU for one hour, washed, fixed with 75 % ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 kappa Isotype Control Left Panel or PE Mouse Anti-BrdU antibody Right Panel; Cat No. 556029 followed by staining with DAPI Solution Cat. No. 564907; nucleic acid stain . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System Two-color flow cytometric analysis of DNA synthesis by REH cells. REH Human leukemia, ATCC CRL-8286 cells were labeled with 1mM BrdU for one hour, washed, fixed with 75 % ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 kappa Isotype Control Left Panel or PE Mouse Anti-BrdU antibody Right Panel; Cat No. 556029 followed by staining with DAPI Solution Cat. No. 564907; nucleic acid stain . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System Show More Two-color flow cytometric analysis of DNA synthesis by REH cells. REH Human leukemia, ATCC CRL-8286 cells were labeled with 1mM BrdU for one hour, washed, fixed with 75 % ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 kappa Isotype Control Left Panel or PE Mouse Anti-BrdU antibody Right Panel; Cat No. 556029 followed by staining with DAPI Solution Cat. No. 564907; nucleic acid stain . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System Show More Two-color flow cytometric analysis of DNA synthesis by REH cells. REH Human leukemia, ATCC CRL-8286 cells were labeled with 1mM BrdU for one hour, washed, fixed with 75 % ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 kappa Isotype Control Left Panel or PE Mouse Anti-BrdU antibody Right Panel; Cat No. 556029 followed by staining with DAPI Solution Cat. No. 564907; nucleic acid stain . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System Two-color flow cytometric analysis of DNA synthesis by REH cells. REH Human leukemia, ATCC CRL-8286 cells were labeled with 1mM BrdU for one hour, washed, fixed with 75 % ice cold ethanol and permeabilized with Tween-20. The cells were then stained with either PE Mouse IgG1 kappa Isotype Control Left Panel or PE Mouse Anti-BrdU antibody Right Panel; Cat No. 556029 followed by staining with DAPI Solution Cat. No. 564907; nucleic acid stain . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System