BD PharmingenTM PE Mouse Anti-c-Maf

Description

Flow cytometric analysis of c-MAF expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC; Left Panel and C57BL/6 mouse peritoneal exudate cells PEC; Right Panel were preincubated with either BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 , or Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , respectively. The cells were then bulk treated with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and washed with TF Perm/Wash. The human PBMC were then stained with Alexa Fluor 700 Mouse anti-Human CD11b antibody Cat. No. 557918 , and either PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Left Plot , or PE Mouse Anti-c-Maf antibody Cat. No. 565795; Right Plot . The mouse PEC were stained with BD Horizon V500 Rat anti-Mouse CD11b antibody, and either PE Mouse IgG2a, kappa Isotype Control Left Plot , or PE Mouse Anti-c-Maf antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD11b versus c-MAF or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact human PBMC or mouse PEC, respectively. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of c-MAF expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC; Left Panel and C57BL/6 mouse peritoneal exudate cells PEC; Right Panel were preincubated with either BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 , or Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , respectively. The cells were then bulk treated with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and washed with TF Perm/Wash. The human PBMC were then stained with Alexa Fluor 700 Mouse anti-Human CD11b antibody Cat. No. 557918 , and either PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Left Plot , or PE Mouse Anti-c-Maf antibody Cat. No. 565795; Right Plot . The mouse PEC were stained with BD Horizon V500 Rat anti-Mouse CD11b antibody, and either PE Mouse IgG2a, kappa Isotype Control Left Plot , or PE Mouse Anti-c-Maf antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD11b versus c-MAF or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact human PBMC or mouse PEC, respectively. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of c-MAF expression in human and mouse leucocytes. Human peripheral blood mononuclear cells PBMC; Left Panel and C57BL/6 mouse peritoneal exudate cells PEC; Right Panel were preincubated with either BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 , or Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , respectively. The cells were then bulk treated with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and washed with TF Perm/Wash. The human PBMC were then stained with Alexa Fluor 700 Mouse anti-Human CD11b antibody Cat. No. 557918 , and either PE Mouse IgG2a, kappa Isotype Control Cat. No. 554648; Left Plot , or PE Mouse Anti-c-Maf antibody Cat. No. 565795; Right Plot . The mouse PEC were stained with BD Horizon V500 Rat anti-Mouse CD11b antibody, and either PE Mouse IgG2a, kappa Isotype Control Left Plot , or PE Mouse Anti-c-Maf antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD11b versus c-MAF or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact human PBMC or mouse PEC, respectively. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.