BD PharmingenTM PE Mouse anti-Doublecortin

Description

LEFT: Analysis of doublecortin DCX in human embryonic stem ES cell-derived neurons. A sorted population of neural stem cells NSC , that had been derived from H9 cells WiCell, Madison, WI , were differentiated in NSC differentiation medium containing N2 and B-27 supplements Life Technologies , recombinant human BDNF and GDNF Peprotech , dibutryl cyclic AMP Sigma for 4 weeks. The neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of a PE Mouse IgG1, kappa isotype control shaded histogram, Cat. No. 554680 or the PE Mouse anti-Doublecortin antibody open histogram . Histograms were derived from gated events based on light scattering characteristics for the H9-derived neurons. The dimmer peak consists of NSC and glial progenitors, and the brighter peak consists of the immature neuron population that is staining positive for doublecortin. RIGHT: Analysis of doublecortin DCX on human ES cell-derived neurons and glia. NSC derived from H9 cells WiCell, Madison, WI were differentiated in NSC differentiation medium for 11 days followed by AGM Astrocyte Growth Medium Lonza for 16 days. The neurons and glia were harvested, fixed and permeabilized, and stained with PE Mouse Anti-Doublecortin and Alexa Fluor 647 Mouse anti-GFAP Cat. No. 561470 monoclonal antibodies. The dot plot was derived from gated events based on light scattering characteristics for the H9-derived constituents. All flow cytometry was performed on a BD LSR II flow cytometry system. LEFT: Analysis of doublecortin DCX in human embryonic stem ES cell-derived neurons. A sorted population of neural stem cells NSC , that had been derived from H9 cells WiCell, Madison, WI , were differentiated in NSC differentiation medium containing N2 and B-27 supplements Life Technologies , recombinant human BDNF and GDNF Peprotech , dibutryl cyclic AMP Sigma for 4 weeks. The neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of a PE Mouse IgG1, kappa isotype control shaded histogram, Cat. No. 554680 or the PE Mouse anti-Doublecortin antibody open histogram . Histograms were derived from gated events based on light scattering characteristics for the H9-derived neurons. The dimmer peak consists of NSC and glial progenitors, and the brighter peak consists of the immature neuron population that is staining positive for doublecortin. RIGHT: Analysis of doublecortin DCX on human ES cell-derived neurons and glia. NSC derived from H9 cells WiCell, Madison, WI were differentiated in NSC differentiation medium for 11 days followed by AGM Astrocyte Growth Medium Lonza for 16 days. The neurons and glia were harvested, fixed and permeabilized, and stained with PE Mouse Anti-Doublecortin and Alexa Fluor 647 Mouse anti-GFAP Cat. No. 561470 monoclonal antibodies. The dot plot was derived from gated events based on light scattering characteristics for the H9-derived constituents. All flow cytometry was performed on a BD LSR II flow cytometry system. LEFT: Analysis of doublecortin DCX in human embryonic stem ES cell-derived neurons. A sorted population of neural stem cells NSC , that had been derived from H9 cells WiCell, Madison, WI , were differentiated in NSC differentiation medium containing N2 and B-27 supplements Life Technologies , recombinant human BDNF and GDNF Peprotech , dibutryl cyclic AMP Sigma for 4 weeks. The neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of a PE Mouse IgG1, kappa isotype control shaded histogram, Cat. No. 554680 or the PE Mouse anti-Doublecortin antibody open histogram . Histograms were derived from gated events based on light scattering characteristics for the H9-derived neurons. The dimmer peak consists of NSC and glial progenitors, and the brighter peak consists of the immature neuron population that is staining positive for doublecortin. RIGHT: Analysis of doublecortin DCX on human ES cell-derived neurons and glia. NSC derived from H9 cells WiCell, Madison, WI were differentiated in NSC differentiation medium for 11 days followed by AGM Astrocyte Growth Medium Lonza for 16 days. The neurons and glia were harvested, fixed and permeabilized, and stained with PE Mouse Anti-Doublecortin and Alexa Fluor 647 Mouse anti-GFAP Cat. No. 561470 monoclonal antibodies. The dot plot was derived from gated events based on light scattering characteristics for the H9-derived constituents. All flow cytometry was performed on a BD LSR II flow cytometry system. Show More LEFT: Analysis of doublecortin DCX in human embryonic stem ES cell-derived neurons. A sorted population of neural stem cells NSC , that had been derived from H9 cells WiCell, Madison, WI , were differentiated in NSC differentiation medium containing N2 and B-27 supplements Life Technologies , recombinant human BDNF and GDNF Peprotech , dibutryl cyclic AMP Sigma for 4 weeks. The neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of a PE Mouse IgG1, kappa isotype control shaded histogram, Cat. No. 554680 or the PE Mouse anti-Doublecortin antibody open histogram . Histograms were derived from gated events based on light scattering characteristics for the H9-derived neurons. The dimmer peak consists of NSC and glial progenitors, and the brighter peak consists of the immature neuron population that is staining positive for doublecortin. RIGHT: Analysis of doublecortin DCX on human ES cell-derived neurons and glia. NSC derived from H9 cells WiCell, Madison, WI were differentiated in NSC differentiation medium for 11 days followed by AGM Astrocyte Growth Medium Lonza for 16 days. The neurons and glia were harvested, fixed and permeabilized, and stained with PE Mouse Anti-Doublecortin and Alexa Fluor 647 Mouse anti-GFAP Cat. No. 561470 monoclonal antibodies. The dot plot was derived from gated events based on light scattering characteristics for the H9-derived constituents. All flow cytometry was performed on a BD LSR II flow cytometry system. Show More LEFT: Analysis of doublecortin DCX in human embryonic stem ES cell-derived neurons. A sorted population of neural stem cells NSC , that had been derived from H9 cells WiCell, Madison, WI , were differentiated in NSC differentiation medium containing N2 and B-27 supplements Life Technologies , recombinant human BDNF and GDNF Peprotech , dibutryl cyclic AMP Sigma for 4 weeks. The neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of a PE Mouse IgG1, kappa isotype control shaded histogram, Cat. No. 554680 or the PE Mouse anti-Doublecortin antibody open histogram . Histograms were derived from gated events based on light scattering characteristics for the H9-derived neurons. The dimmer peak consists of NSC and glial progenitors, and the brighter peak consists of the immature neuron population that is staining positive for doublecortin. RIGHT: Analysis of doublecortin DCX on human ES cell-derived neurons and glia. NSC derived from H9 cells WiCell, Madison, WI were differentiated in NSC differentiation medium for 11 days followed by AGM Astrocyte Growth Medium Lonza for 16 days. The neurons and glia were harvested, fixed and permeabilized, and stained with PE Mouse Anti-Doublecortin and Alexa Fluor 647 Mouse anti-GFAP Cat. No. 561470 monoclonal antibodies. The dot plot was derived from gated events based on light scattering characteristics for the H9-derived constituents. All flow cytometry was performed on a BD LSR II flow cytometry system. LEFT: Analysis of doublecortin DCX in human embryonic stem ES cell-derived neurons. A sorted population of neural stem cells NSC , that had been derived from H9 cells WiCell, Madison, WI , were differentiated in NSC differentiation medium containing N2 and B-27 supplements Life Technologies , recombinant human BDNF and GDNF Peprotech , dibutryl cyclic AMP Sigma for 4 weeks. The neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of a PE Mouse IgG1, kappa isotype control shaded histogram, Cat. No. 554680 or the PE Mouse anti-Doublecortin antibody open histogram . Histograms were derived from gated events based on light scattering characteristics for the H9-derived neurons. The dimmer peak consists of NSC and glial progenitors, and the brighter peak consists of the immature neuron population that is staining positive for doublecortin. RIGHT: Analysis of doublecortin DCX on human ES cell-derived neurons and glia. NSC derived from H9 cells WiCell, Madison, WI were differentiated in NSC differentiation medium for 11 days followed by AGM Astrocyte Growth Medium Lonza for 16 days. The neurons and glia were harvested, fixed and permeabilized, and stained with PE Mouse Anti-Doublecortin and Alexa Fluor 647 Mouse anti-GFAP Cat. No. 561470 monoclonal antibodies. The dot plot was derived from gated events based on light scattering characteristics for the H9-derived constituents. All flow cytometry was performed on a BD LSR II flow cytometry system.