BD PharmingenTM PE Mouse anti-GFAP

Description

Analysis of GFAP in differentiated human Neural Stem Cells NSC . NSC derived from H9 cells WiCell, Madison, WI were differentiated in NSC differentiation medium containing N2 and B-27 supplements Life Technologies , recombinant human BDNF and GDNF Peprotech , dibutryl cyclic AMP Sigma for 11 days followed by AGM Astrocyte Growth Medium Lonza for 16 days. The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No.557885 , and then stained with PE Mouse anti-GFAP left panel and co-stained with APC mouse anti-CD44 Cat. No. 559942 as shown in the right panel. This antibody conjugate also works with BD Phosflow Perm Buffer III. Flow cytometry was performed on a BD LSR II flow cytometer. Analysis of GFAP in Neural Stem cells NSC . NSC were isolated by sorting from Embryoid bodies and were grown for 8 passages post sort, fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No.557885 , and then stained with either PE Mouse anti-GFAP solid line or PE Mouse IgG 2b , kappa Isotype Control Cat. No. 555058, dashed line . Analysis of GFAP in differentiated human Neural Stem Cells NSC . NSC derived from H9 cells WiCell, Madison, WI were differentiated in NSC differentiation medium containing N2 and B-27 supplements Life Technologies , recombinant human BDNF and GDNF Peprotech , dibutryl cyclic AMP Sigma for 11 days followed by AGM Astrocyte Growth Medium Lonza for 16 days. The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No.557885 , and then stained with PE Mouse anti-GFAP left panel and co-stained with APC mouse anti-CD44 Cat. No. 559942 as shown in the right panel. This antibody conjugate also works with BD Phosflow Perm Buffer III. Flow cytometry was performed on a BD LSR II flow cytometer. Show More Analysis of GFAP in Neural Stem cells NSC . NSC were isolated by sorting from Embryoid bodies and were grown for 8 passages post sort, fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No.557885 , and then stained with either PE Mouse anti-GFAP solid line or PE Mouse IgG 2b , kappa Isotype Control Cat. No. 555058, dashed line . Show More Analysis of GFAP in differentiated human Neural Stem Cells NSC . NSC derived from H9 cells WiCell, Madison, WI were differentiated in NSC differentiation medium containing N2 and B-27 supplements Life Technologies , recombinant human BDNF and GDNF Peprotech , dibutryl cyclic AMP Sigma for 11 days followed by AGM Astrocyte Growth Medium Lonza for 16 days. The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No.557885 , and then stained with PE Mouse anti-GFAP left panel and co-stained with APC mouse anti-CD44 Cat. No. 559942 as shown in the right panel. This antibody conjugate also works with BD Phosflow Perm Buffer III. Flow cytometry was performed on a BD LSR II flow cytometer. Analysis of GFAP in Neural Stem cells NSC . NSC were isolated by sorting from Embryoid bodies and were grown for 8 passages post sort, fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No.557885 , and then stained with either PE Mouse anti-GFAP solid line or PE Mouse IgG 2b , kappa Isotype Control Cat. No. 555058, dashed line .