BD PharmingenTM PE Mouse Anti-Glucagon

Description

Flow cytometric analysis of Glucagon expression in human Glucagon-transfected 293F cells and a mouse pancreas alpha cell line. LEFT PANEL: Untransfected dashed-line histogram and human Glucagon-transfected solid-line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with PE Mouse Anti-Glucagon Cat. No. 565860 . RIGHT PANEL: Alpha TC1-6 cells ATCC CRL-2934 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with either PE Mouse IgG1 kappa Isotype control Cat. No. 554680, dashed-line histogram or PE Mouse Anti-Glucagon Cat. No. 565860, solid-line histogram . All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Flow cytometric analysis of Glucagon expression in human Glucagon-transfected 293F cells and a mouse pancreas alpha cell line. LEFT PANEL: Untransfected dashed-line histogram and human Glucagon-transfected solid-line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with PE Mouse Anti-Glucagon Cat. No. 565860 . RIGHT PANEL: Alpha TC1-6 cells ATCC CRL-2934 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with either PE Mouse IgG1 kappa Isotype control Cat. No. 554680, dashed-line histogram or PE Mouse Anti-Glucagon Cat. No. 565860, solid-line histogram . All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Flow cytometric analysis of Glucagon expression in human Glucagon-transfected 293F cells and a mouse pancreas alpha cell line. LEFT PANEL: Untransfected dashed-line histogram and human Glucagon-transfected solid-line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with PE Mouse Anti-Glucagon Cat. No. 565860 . RIGHT PANEL: Alpha TC1-6 cells ATCC CRL-2934 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with either PE Mouse IgG1 kappa Isotype control Cat. No. 554680, dashed-line histogram or PE Mouse Anti-Glucagon Cat. No. 565860, solid-line histogram . All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Show More Flow cytometric analysis of Glucagon expression in human Glucagon-transfected 293F cells and a mouse pancreas alpha cell line. LEFT PANEL: Untransfected dashed-line histogram and human Glucagon-transfected solid-line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with PE Mouse Anti-Glucagon Cat. No. 565860 . RIGHT PANEL: Alpha TC1-6 cells ATCC CRL-2934 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with either PE Mouse IgG1 kappa Isotype control Cat. No. 554680, dashed-line histogram or PE Mouse Anti-Glucagon Cat. No. 565860, solid-line histogram . All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Show More Flow cytometric analysis of Glucagon expression in human Glucagon-transfected 293F cells and a mouse pancreas alpha cell line. LEFT PANEL: Untransfected dashed-line histogram and human Glucagon-transfected solid-line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with PE Mouse Anti-Glucagon Cat. No. 565860 . RIGHT PANEL: Alpha TC1-6 cells ATCC CRL-2934 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with either PE Mouse IgG1 kappa Isotype control Cat. No. 554680, dashed-line histogram or PE Mouse Anti-Glucagon Cat. No. 565860, solid-line histogram . All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Flow cytometric analysis of Glucagon expression in human Glucagon-transfected 293F cells and a mouse pancreas alpha cell line. LEFT PANEL: Untransfected dashed-line histogram and human Glucagon-transfected solid-line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with PE Mouse Anti-Glucagon Cat. No. 565860 . RIGHT PANEL: Alpha TC1-6 cells ATCC CRL-2934 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and then stained with either PE Mouse IgG1 kappa Isotype control Cat. No. 554680, dashed-line histogram or PE Mouse Anti-Glucagon Cat. No. 565860, solid-line histogram . All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system.