BD PharmingenTM PE Mouse Anti-Human Basophils (2D7)

Description

Multiparameter flow cytometric analysis of Basophils 2D7 expression on human peripheral blood leucocyte populations. Human whole blood was fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , and the cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Basophils 2D7 antibody Cat. No. 568076/568077; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Basophils 2D7 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of Basophils 2D7 expression on human peripheral blood leucocytes. Human whole blood was stained with BV421 Mouse Anti-Human CD193 antibody Cat. No. 562570 and then fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Basophils 2D7 antibody Cat. No. 568076/568077; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Basophils 2D7 or Ig Isotype control staining versus CD193 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of Basophils 2D7 expression on human peripheral blood leucocyte populations. Human whole blood was fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , and the cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Basophils 2D7 antibody Cat. No. 568076/568077; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Basophils 2D7 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of Basophils 2D7 expression on human peripheral blood leucocytes. Human whole blood was stained with BV421 Mouse Anti-Human CD193 antibody Cat. No. 562570 and then fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Basophils 2D7 antibody Cat. No. 568076/568077; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Basophils 2D7 or Ig Isotype control staining versus CD193 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of Basophils 2D7 expression on human peripheral blood leucocyte populations. Human whole blood was fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , and the cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Basophils 2D7 antibody Cat. No. 568076/568077; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Basophils 2D7 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of Basophils 2D7 expression on human peripheral blood leucocytes. Human whole blood was stained with BV421 Mouse Anti-Human CD193 antibody Cat. No. 562570 and then fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 and permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . The cells were then stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plot or PE Mouse Anti-Human Basophils 2D7 antibody Cat. No. 568076/568077; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Basophils 2D7 or Ig Isotype control staining versus CD193 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.