BD PharmingenTM PE Mouse Anti-Human C3b/iC3b

Description

Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Left Plot; Anti-CD3 + Serum or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Right Plot; Isotype Control + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histograms or with PE Mouse Anti-Human C3b/iC3b antibody Cat. No. 567548/567583; solid line histograms . The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Left Plot; Anti-CD3 + Serum or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Right Plot; Isotype Control + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histograms or with PE Mouse Anti-Human C3b/iC3b antibody Cat. No. 567548/567583; solid line histograms . The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Left Plot; Anti-CD3 + Serum or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Right Plot; Isotype Control + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histograms or with PE Mouse Anti-Human C3b/iC3b antibody Cat. No. 567548/567583; solid line histograms . The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Left Plot; Anti-CD3 + Serum or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Right Plot; Isotype Control + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histograms or with PE Mouse Anti-Human C3b/iC3b antibody Cat. No. 567548/567583; solid line histograms . The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Left Plot; Anti-CD3 + Serum or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Right Plot; Isotype Control + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histograms or with PE Mouse Anti-Human C3b/iC3b antibody Cat. No. 567548/567583; solid line histograms . The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of C3b/iC3b fixed on antibody and complement treated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were preincubated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336; Left Plot; Anti-CD3 + Serum or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645; Right Plot; Isotype Control + Serum , pelleted by centrifugation, and then resuspended in human serum for a source of complement for 30 min at 37C as indicated. After washing, the cells were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dotted line histograms or with PE Mouse Anti-Human C3b/iC3b antibody Cat. No. 567548/567583; solid line histograms . The histograms showing bound levels of C3b/iC3b or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.