
561701 · 25 Tests
BDB Bioscience · Cat: 561701
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD137 expression on viable stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human CD137 antibody Cat. No. 555956/561701; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD137 expression on viable stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human CD137 antibody Cat. No. 555956/561701; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD137 expression on viable stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either PE Mouse IgG1, kappa Isotype Control Cat. No. 554680; dashed line histogram or PE Mouse Anti-Human CD137 antibody Cat. No. 555956/561701; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.